抗H2AX抗体 | Anti-H2AX Antibody

掲載日情報:2018/07/09 現在Webページ番号:46904

世界最大級の抗体製品数を取り扱うNovus Biologicals社のH2AXに対する抗体(anti-H2AX | antibody H2AX)です。Novus Biologicals社の抗体は数多くの学術論文で使用実績があります。

本製品は研究用です。研究用以外には使用できません。

価格表左の「文献」アイコンから、使用文献情報一覧が表示できます。

価格

[在庫・価格 :2025年04月27日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-H2AX, Rabbit-Poly
3~4週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 10
説明文
抗原:ヒトhistone H2AX C-terminal付近の配列を持つ合成ペプチド,Keywords:BTF antibHistone H2AX|Gamma H2AX|H2A histone family member X|H2A.FX|H2A.X|H2A/X|H2AFX|H2AX|H2AX histone|Hist5.2ax|Histone 2A|Histone 2A.X|Histone 2AX|Histone H2A.x|Histone H2A/X|Histone H2AFX|Histone H2AX|Including osteosarcoma|OSRC|P105 RB|P105RB|PP105
Genbank No: 3014
Protein Accession No: P16104
法規制等
保存条件 4℃,凍結禁止 法規備考
抗原種 Human 免疫動物 Rabbit クラス IgG 標識 Unlabeled
交差性 Human/Mouse 適用 IC,IF,IHC,IP,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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[在庫・価格 :2025年04月27日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-H2AX, Rabbit-Poly

文献数: 10

説明文 抗原:ヒトhistone H2AX C-terminal付近の配列を持つ合成ペプチド,Keywords:BTF antibHistone H2AX|Gamma H2AX|H2A histone family member X|H2A.FX|H2A.X|H2A/X|H2AFX|H2AX|H2AX histone|Hist5.2ax|Histone 2A|Histone 2A.X|Histone 2AX|Histone H2A.x|Histone H2A/X|Histone H2AFX|Histone H2AX|Including osteosarcoma|OSRC|P105 RB|P105RB|PP105
Genbank No: 3014
Protein Accession No: P16104
法規制等
保存条件 4℃,凍結禁止 法規備考
抗原種 Human 免疫動物 Rabbit
交差性 Human/Mouse 適用 IC,IF,IHC,IP,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事



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Image

Western Blot: H2AX Antibody [NB100-383] - Samples: Whole cell lysate (15 ug) from NIH3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: H2AX Antibody [NB100-383] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Biological Strategies Validation. Western Blot: H2AX Antibody [NB100-383] - Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3.
Biological Strategies Validation. Western Blot: H2AX Antibody [NB100-383] - Human 293T, human G-361, and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at 0.1 ug/ml.
Biological Strategies Validation. Western Blot: H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from 293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: H2AX Antibody [NB100-383] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anit-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human H2AFX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human H2AX (NB100-383). Image merged from DAPI (2ms), FITC/Green ( 25ms) and TRITC/Orange (250ms) exposures, 40X magnification.
Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RAD17 (NB100-272) and rabbit anti-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: H2AX Antibody [NB100-383] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human H2AX (NB100-383). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Simple Western: H2AX Antibody [NB100-383] - Simple Western lane view shows a specific band for H2AX in 0.2 mg/ml of HEK lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.
Simple Western: H2AX Antibody [NB100-383] - Electropherogram image(s) of corresponding Simple Western lane view. H2AX antibody was used at 10 ug/ml dilution on HEK lysate(s).


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Background

Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.

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