抗PHD4/HIF Prolyl Hydroxylase 4抗体 | Anti-PHD4/HIF Prolyl Hydroxylase 4 Antibody

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	Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
	Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Analysis of human PHD4, using NB100-295. Samples: whole cell lysate from HeLa cells, normoxic and hypoxic, respectively.
	Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [NB100-295] - Whole cell lysate (25 mcg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4. Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. NB100-295) used at 2 mcg/ml. Detection: Chemiluminescence

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Background

HIF prolyl 4-hydroxylases (HIF-P4Hs or PHDs) are proyl hydroxylase domain-containing enzymes (PHD1, PHD2, PHD3, and P4H-TM) that mediate key physiological responses to hypoxia by modulating HIF1 alpha levels. PHD member proteins have a wide but varied tissue expression and PHD4/HIF Prolyl Hydroxylase 4 (P4H-TM) is a novel member of this family that localize to ER membrane as single-pass type II membrane protein with its catalytic site inside the lumen. P4H-TM is expressed mainly in pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland tissues and gets induced upon hypoxic trigger. P4H-TM catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins and hydroxylates HIF1A at 'Pro-402'/'Pro-564' sites. P4H-TM has been proposed to function as a cellular oxygen sensor and, under normoxic conditions, it targets HIF through hydroxylation for proteasomal degradation via VHL/von Hippel-Lindau ubiquitination complex. Ectopic over-expression of P4H-TM in cultured neuroblastoma cells was found to reduce HIF-1alpha ODDD reporter levels, whereas its knockdown increased HIF-1alpha protein levels. HIF-P4Hs plays a critical role in several pathologies such as myocardial infarction, stroke, peripheral vascular disease, inflammation, diabetes, carcinogenesis and severe anemias.

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