抗p70 S6 Kinase/S6K抗体 | Anti-p70 S6 Kinase/S6K Antibody

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Biological Strategies Validation. Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Human and Mouse p70S6K by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p70S6K antibody NB100-293 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Biological Strategies Validation. Western Blot: p70 S6 Kinase/S6K Antibody [NB100-293] - Detection of Rat p70S6 Kinase by Western Blot. Sample: Lysate from rat L6 myoblast that were serum starved for 1.5 h (lane 1) or serum-deprived for 1 h and then treated with 20 ng/ml IGF-1 for 30 minutes (lane 2). Antibody: Affinity purified rabbit anti-p70S6 kinase (NB100-293) used at 0.1 mcg/ml. Detection: Chemiluminescence.

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Background

Ribosomal S6 kinase has two isoforms, termed S6K1 (S6Ka) and S6K2 (S6Kb). The S6K1 isoform has two splicing forms, which are the products of alternative start codon usage. The first splicing form, p70S6K or p70S6K1, has a predicted molecular mass of 56 kDa and an apparent molecular mass (SDS-PAGE) of 70 kDa. The second isoform, p85S6K or p85S6K1, contains 23 extra amino acid residues at the N terminus, resulting from the alternative start codon. It has a predicted molecular mass of 59 kDa and an apparent molecular mass of 85 kDa. Both, p70 and p85 S6K1 are activated by multiple S/T phosphorylations in response to various mitogenic stimuli and nutrients. This is accompanied by significant mobility shift in the migration of both forms, when analysed by SDS-PAGE electrophoresis.

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