抗DNMT1抗体 | Anti-DNMT1 Antibody

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	Genetic Strategies Validation. Knockout Validated: DNMT1 Antibody [NB100-264] - Western blot shows lysates of Hek293 human embryonic kidney parental cell line and DNMT1 knockout (KO) Hek293 cell line. PVDF membrane was probed with 1:100 of Rabbit Anti-Human DNMT1 Polyclonal Antibody (Catalog # NB100-264) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for DNMT1 at approximately 240 kDa (as indicated) in the parental Hek293 cell line, but is not detectable in the knockout Hek293 cell line. This experiment was conducted under reducing conditions.
	Western Blot: DNMT1 Antibody [nb100-264] - DNMT1 in Fibroblasts. 30ug of whole cell lysate. This image was submitted via customer Review.
	Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [nb100-264] - Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [NB100-264] - The Dnmt1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
	Immunohistochemistry-Paraffin: DNMT1 Antibody [nb100-264] - Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - IHC staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.
	Western Blot: DNMT1 Antibody [nb100-264] - Western Blot: DNMT1 Antibody [NB100-264] - Analysis using NB100-264 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ugs of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ugs of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.
	Immunohistochemistry-Paraffin: DNMT1 Antibody [nb100-264] - Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - IHC staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.
	Simple Western: DNMT1 Antibody [nb100-264] - Simple Western: DNMT1 Antibody [NB100-264] - Simple Western lane view shows a specific band for Dnmt1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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Background

Member of C5-methyltransferase family and the most abundant DNA methyltransferase in mammalian cells, Dnmt1 (DNA methyltransferase 1) is a key methylation enzyme implicated in maintaining methylation during DNA replication/repair which has capability of methylating CpG residues with preference to hemimethylated DNA and de novo methylation during somatic cell development/differentiation. Dnmt1 interacts with CSNK1D, HDAC1, PCNA, MBD2, MBD3, DNMT3A, DNMT3B, PRC2/EED-EZH2 complex, UBC9, BAZ2A/TIP5, UHRF1, USP7 etc., and forms complexes with DMAP1, HDAC2, E2F1, BB1, HDAC1 and SUV39H1. In cell division, Dnmt1 associates with DNA replication sites in S phase maintaining the methylation pattern in newly synthesized strand, that is essential for epigenetic inheritance. Moreover, it associates with chromatin during G2 as well as M phases to maintain DNA methylation independently of replication, and is reduced to non detectable levels at the G0 phase with dramatic induction upon entry into S-phase. Dnmt1 mediates transcriptional repression by direct binding to HDAC2 and in association with DNMT3B/via the recruitment of CTCFL/BORIS, it involves in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 as well as H3K9. Dnmt1 is responsible for maintaining methylation patterns established in development and defects in DNMT1 are the cause of hereditary sensory neuropathy type 1E (HSN1E).

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