抗RNA Polymerase II/POLR2A [p Ser2]抗体 | Anti-RNA Polymerase II/POLR2A [p Ser2] Antibody

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	Biological Strategies Validation. Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.
	Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Samples: FFPE serial sections of human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:500 (
	Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Hela whole cell lysate (50 ug each lane) loaded and probed by anti-RNA polymerase II Antibody. NB100-1805 was pre-incubated without peptide (-), with non-phosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide.
	Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.08 ug/ml.
	Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-RNA Polymerase II (S2). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
	Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Mouse Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-RNA Polymerase II (S2). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
	Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Analysis of POLR2A in MCF-7 xenograft using anti-POLR2A [p Ser2] antibody. Image from verified customer review.
	Proximity Ligation Assay: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human phospho-POLR2A {S2] (NB100-1805). Image merged from DAPI (2ms), FITC/Green (25ms) and TRITC/Orange (250) exposures, 40X magnification.
	Proximity Ligation Assay: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM3 (NB100-249) and rabbit anti-human phospho-POLR2A [S2] (NB100-1805). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
	Proximity Ligation Assay: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1(NB100-287) and rabbit anti-human phospho-POLR2A [S2] (NB100-1805). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
	Proximity Ligation Assay: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Secondary-conjugate Duolink II PLA in Hela cells. goat anit-human FUS (NBP2-14860) and rabbit anti-human phospho-POLR2A [S2] (NB100-1805). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

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Background

The RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II core complex. RNA polymerase II holoenzyme functions to transcribe DNA into messenger RNA in eukaryotes. RPB1 forms the DNA binding domain of the polymerase that forms a cleft that grabs the incoming DNA template to translocate the holoenzyme across the RNA-DNA hybrid. Alternate identifiers of RPB1 include DNA-directed RNA polymerase II subunit RPB1, DNA directed RNA polymerse II subunit A, DNA-directed RNA polymerase II largest subunit, POLR2A, RPO2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220, and MGC75453.

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