抗GFP抗体 | Anti-GFP Antibody

• 価格
• Image
• Background

価格

目次に戻る

Image

	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - Analysis using the Biotin conjugate of NB100-1770. Staining of transgenic mouse pancreas, expressing GFP in beta cells. Image courtesy of product review by Dr. Yves Heremans of Vrije Universiteit Brussel.
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - Analysis of Biotin conjugate of NB100-1770. Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-GFP antibody at a 1:1,000 for 1 h at RT. Secondary antibody.
	Genetic Strategies Validation. Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - Analysis using the Biotin conjugate of NB100-1770. Staining of GFP in mouse aorta, liver, brain, and lung cells. Image courtesy of product review by Laura Shankman.
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - Antibody. Tissue: E5.5 Hex-GFP transgenic mouse embryo. Primary antibody: Goat anti-GFP was used at 1:500 dilution. Secondary antibody: Fluorochrome conjugated Anti-goat IgG secondary antibody at 1:10,000 for 45 min at RT. Staining: GFP as green fluorescent signal with DAPI blue counterstain.
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - IF analysis of GFP in mouse brain. Image courtesy of product review by Tatyana Pivneva.
	Western Blot: GFP Antibody [NB100-1770] - Analysis using the Alkaline Phosphatase conjugate of NB100-1770. Detection of Lane 1: GFP. Detection of Lane 2: none. Load: 50 ng per lane. Alkaline Phosphatase GFP secondary antibody at 1:1000 for 60 min at RT. Predicted/Observed size: 28 kDa for GFP.
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - HEK293 cells stable transfected with integrin alpha8-GFP, were immunostained for GFP (green) and counterstained with phalloidin (red) and DAPI (BLUE). Image from verified customer review.
	Immunohistochemistry: GFP Antibody [NB100-1770] - GFP-positive transplanted NPCs have morphological features of hippocampal pyramidal neurons at day 90 after grafting. Image from verified customer review.
	Western Blot: GFP Antibody [NB100-1770] - Comparison of fluorescence absorption and emission spectra for DyLight (TM) 488 and Alexa Fluor 488 in PBS, pH7.2. The emission spectra for this DyLight (TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.
	Western Blot: GFP Antibody [NB100-1770] - Analysis using the FITC conjugate of NB100-1770. Detection of Lane 1: GFP. Detection of Lane 2: None. Load: 50ug per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 mi
	Western Blot: GFP Antibody [NB100-1770] - Analysis using Biotin conjugate of NB100-1770. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. Primary antibody: GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 28 kDa, 28 kDa for GFP. Other band(s): None.
	Western Blot: GFP Antibody [NB100-1770] - Analysis using Biotin conjugate of NB100-1770. Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. Primary antibody: GFP antibody at 1 ug/ml for 1 h at room temperature. Secondary antibody: IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 secondary antibody at 1:2,500 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kDa, 33 kDa for GFP. Other band(s): none.
	Western Blot: GFP Antibody [NB100-1770] - Analysis using HRP conjugate of NB100-1770. Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Peroxidase Conjugate secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS 30 min at 20C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - Analysis using the Biotin conjugate of NB100-1770. Staining of GFP-Hela cells against a Streptavidin conjugated 550 secondary. Hela-GFP are illuminated in Green and nuclei in Blue (DAPI).
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - Tissue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Primary antibody: Goat anti-GFP was used at 1:500 dilution in free floating immunohistochemistry to detect GFP. Secondary antibody: Fluorochrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.
	Immunocytochemistry/Immunofluorescence: GFP Antibody [NB100-1770] - Analysis of DyLight 488 conjugate of NB100-1770. Tissue: human breast carcinoma. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Anti-Histone and Anti-Tubulin antibody at 10 ug/mL for 1 h at RT. Secondary antibody: DyLight (R) 488 c
	ELISA: GFP Antibody [NB100-1770] - Analysis using the DyLight 488 conjugate of NB100-1770. Detection of Lane 1: His-Sumo-GFP. Lane: Molecular Weight. Detection of Lane 2: Beta-Galactosidase (negative control). Detection of Lane 3: recombinant GFP control protein. Load: 35 ug per lane. Primary antibody: none. Secondary antibody: DyLight (TM) 488 conjugated anti-GFP goat secondary antibody at 1:5,000. Block: MB-070 for 2 hr at RT. Predicted/Observed size: 27kDa/54kDa, 27kDa for rGFP/~45kDa His-Sumo-GFP.

目次に戻る

Background

Green fluorescence protein (GFP) is a 27 KDa protein derived from the jellyfish Aquorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

目次に戻る