抗Luciferase抗体 | Anti-Luciferase Antibody

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	Western Blot: Luciferase Antibody [NB100-1677] - Lane 1: Luciferase, 50ng.Luciferase antibody at 1:1000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 60 kDa for Luciferase. Other band(s): None.
	Immunocytochemistry/Immunofluorescence: Luciferase Antibody [NB100-1677] - Human tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain. Image provided via product review by verified customer.
	Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase expression in mouse brain tissue bearing human glioma cells expressing fire fly luciferase. Image from verified customer review.
	Western Blot: Luciferase Antibody [NB100-1677]
	Immunocytochemistry/Immunofluorescence: Luciferase Antibody [NB100-1677] - Luciferase in 247Nluc+ cells. Image provided via product review by verified customer.
	Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - NIH3T3 cells. Cells (25,000/well) transfected with pLuc plasmid were stained with 20ug/ml NB100-1677 and 1:200 dilution of donkey anti-goat IgG-FITC (green). Cells were mounted with DAPI (blue) and visualized at 200X magnification.
	Immunohistochemistry-Paraffin: Luciferase Antibody [NB100-1677] - Luciferase immunostaining was analyzed in tibias from ovx ERE-luciferase mice taken 24 h after a 17-E2 injection. Positive luciferase staining was identified in hypertrophic chondrocytes (hc) (A), lining cells (lc) (B), osteoblasts (ob) (C), a subpopulation (10%) of osteocytes (ot) (upper arrow points at a positively stained osteocyte, whereas the lower depicts a negative osteocyte) (D), and megakaryocytes (E). Faint staining was found on the periosteal surface (p) (F). No background staining was seen when omitting the primary antibody (data not shown). The bar in the lower left corner represents 25 um. pc, Proliferative chondrocyte.

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Luciferase is a reporter enzyme that catalyzes a bioluminescent reaction involving the enzyme, Mg2+ and ATP. This reaction produces a brief flash of light that can be detected by a luminometer. The amount of light emitted is directly proportional to the activity of luciferase, making luciferase an excellent tool for studying gene expression.

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