抗Nbs1抗体 | Anti-Nbs1 Antibody

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	Western Blot: Nbs1 Antibody [NB100-143] - TIG-1 human primary fibroblasts, whole cell lysate (30 ug). Image from verified customer review.
	Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - Hela cells. Image from verified customer review.
	Immunohistochemistry-Paraffin: Nbs1 Antibody [NB100-143] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human testis using Nbs1 antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
	Western Blot: Nbs1 Antibody [NB100-143] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.
	Genetic Strategies Validation. Western Blot: Nbs1 Antibody [NB100-143] - Detection of NBS1 in Human Bone Osteosarcoma Epithelial Cells (U2OS whole cell lysate) using NB100-143 at a dilution of 1/1000. Specificity was confirmed using a siRNA anti-NBS1 (targeting sequence: GGAAGAAACGUGAACUCAA). Image provided by Sebastien Britton of the Institute of Pharmacology and Structural Biology.
	Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [NB100-143] - Staining of Hela cells using NB100-143 anti-NBS1.

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Background

NBS1 (Nijmegen breakage syndrome protein 1) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. NBS1's FHA domain binds phospho-Thr residues in Ser-X-Thr motifs present in DNA damage proteins, including Mdc1 and Ctp1, whereas, BRCT domains of NBS1 bind Ser-X-Thr motifs when Ser residue is phosphorylated. These phospho-dependent interactions are important for recruiting repair and checkpoint proteins to DSB sites and NBS1 is responsible for MRN complex's nuclear translocation. NBS1 itself does not possess enzymatic activity and contributes to DSB repair primarily by mediating protein-protein interactions at DNA breakage sites. The central region of NBS1 1 possesses several SQ motifs that are phosphorylated by ATM kinase via DNA damage response. NBS1's C-terminus contains a domain that interacts with ATM and recruits it to DSBs, and induces apoptosis in response to damage. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis.

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