抗ARNT/HIF-1 beta (H1beta234)抗体 | Anti-ARNT/HIF-1 beta (H1beta234) Antibody

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	Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Analysis of HIF-1 beta in HeLa nuclear extract.
	Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HIF-1 beta (H1beta234) at 5ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
	Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta.

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Background

HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits which are bHLH proteins of PAS (PER, ARNT, SIM) family. HIF-1 beta forms heterodimer with AHR, AHRR, HIF1 alpha and EPAS1/HIF2 alpha as well as with other bHLH proteins and its dimerization is required for efficient DNA binding. It has been shown to interact with TACC3 and NOCA7 also. HIF-1 beta is also known as ARNT (AhR nuclear translocator) due to its ability to partner with AhR to form a heterodimeric transcription factor complex which is required for AhR activity. It is essential for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding and the AhR-HIF-1 beta complex then initiates transcription of genes involved in xenobiotic transformation, especially of PAHs. HIF-1 beta heterodimer with HIF1 alpha or EPAS1/HIF2 alpha functions as a transcriptional regulator of the adaptive response to hypoxia. Moreover, a chromosomal translocation resulting in TEL-ARNT fusion protein is associated with AML (acute myeloblastic leukemia) and decreased HIF-1 beta expression levels in pancreatic islets from NIDDM subjects reflects its important role in beta-cell function.

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