抗Human Dectin-1/CLEC7A Phycoerythrin抗体(Anti-Human Dectin-1/CLEC7A Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7AThr66-Met201Accession # NP_072092
Source	Monoclonal Mouse IgG2B Clone # 259931
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Dectin‑1/CLEC7A PE-conjugated Monoclonal Antibody (Catalog # FAB1859P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Dectin-1/CLEC7A

background_content

Background: Dectin-1/CLEC7A Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

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Citations

1. Isoform localization of Dectin-1 regulates the signaling quality of anti-fungal immunity
   Authors: M Fischer, JP Müller, B Spies-Weis, C Gräfe, O Kurzai, K Hünniger, A Hochhaus, S Scholl, U Schnetzke
   Eur. J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans.
   PLoS ONE, 2015;10(3):e0119244.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Opposite regulatory effects of IFN-beta and IL-3 on C-type lectin receptors, antigen uptake, and phagocytosis in human macrophages.
   Authors: Cardone M, Ikeda K, Varano B, Belardelli F, Millefiorini E, Gessani S, Conti L
   J Leukoc Biol, 2014;95(1):161-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.
   Authors: Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M
   PLoS ONE, 2013;8(12):e82734.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. MBL-mediated opsonophagocytosis of Candida albicans by human neutrophils is coupled with intracellular Dectin-1-triggered ROS production.
   Authors: Li D, Dong B, Tong Z, Wang Q, Liu W, Wang Y, Liu W, Chen J, Xu L, Chen L, Duan Y
   PLoS ONE, 2012;7(12):e50589.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Vitamin D(3) down-regulates proinflammatory cytokine response to Mycobacterium tuberculosis through pattern recognition receptors while inducing protective cathelicidin production.
   Authors: Khoo AL, Chai LY, Koenen HJ, Oosting M, Steinmeyer A, Zuegel U, Joosten I, Netea MG, van der Ven AJ
   Cytokine, 2011;55(2):294-300.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
   Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
   Immunol. Lett., 2011;134(2):113-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Dectin-1 Y238X polymorphism associates with susceptibility to invasive aspergillosis in hematopoietic transplantation through impairment of both recipient- and donor-dependent mechanisms of antifungal immunity.
   Authors: Cunha C, Di Ianni M, Bozza S, Giovannini G, Zagarella S, Zelante T, D'Angelo C, Pierini A, Pitzurra L, Falzetti F, Carotti A, Perruccio K, Latge JP, Rodrigues F, Velardi A, Aversa F, Romani L, Carvalho A
   Blood, 2010;116(24):5394-402.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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