抗IL-17抗体(Anti-IL-17, Goat-Poly, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant mouse IL‑17Thr22-Ala158Accession # Q62386
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-17A and recombinant mouse (rm) IL-17F is observed and less than 1% cross-reactivity with rmIL-17B, rmIL-17C, rmIL‑17D, and rmIL-17E is observed.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑17/IL‑17A in ActivatedEL‑4 Mouse Cell Line by Flow Cytometry. Activated EL‑4 mouse lymphoblast cell line was stained with Goat Anti-MouseIL‑17/IL‑17A PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC421P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Interleukin 17
Background	IL-17/IL-17A
background_content	Background: IL-17/IL-17A Interleukin 17, also known as IL-17A and CTLA-8, was initially identified as a 17 kDa, secreted T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 25 amino acid residue signal peptide that is cleaved to yield the 133 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with IL-17F. At the amino acid level, mIL-17 shows 62% and 87% aa sequence identity with human and rat IL-17, respectively. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combinationof IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with LTi cells, B cells, gamma δ T cells, CD4+ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8+ Tc17 cells. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity, and the induction of antimicrobial peptides by epithelium.

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Citations

1. MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis
   Eur. J. Immunol, 2016;0(0):.
   Species: Rat
   Sample Type: Whole Cells
   Application: Flow


2. Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge.
   Authors: Walker C, Hautefort I, Dalton J, Overweg K, Egan C, Bongaerts R, Newton D, Cruickshank S, Andrew E, Carding S
   PLoS ONE, 2013;8(12):e84553.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Demyelination caused by the copper chelator cuprizone halts T cell mediated autoimmune neuroinflammation.
   Authors: Mana P, Fordham SA, Staykova MA, Correcha M, Silva D, Willenborg DO, Linares D
   J. Neuroimmunol., 2009;210(1):13-21.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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