抗IL-31RA抗体(Anti-IL-31RA, Goat-Poly, PE antibody)

掲載日情報:2018/11/26 現在Webページ番号:33943

IL-31RAに対する抗体(Anti-IL-31RA, Goat-Poly, PE )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-IL-31RA, Goat-Poly, PE (100tests)
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説明文
別名:class I cytokine receptor
Genbank No: 133396
Protein Accession No: Q8NI17
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 PE
交差性 Human 適用 FCM
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
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[在庫・価格 :2024年06月08日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-IL-31RA, Goat-Poly, PE (100tests)

文献数: 1

説明文 別名:class I cytokine receptor
Genbank No: 133396
Protein Accession No: Q8NI17
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 FCM
標識 PE 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
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Product Details

Species ReactivityHuman
LabelPhycoerythrin
ImmunogenMouse myeloma cell line NS0-derived recombinant human IL-31 RAAla20-Ser516Accession # Q8NI17
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human IL-31 RA in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse IL‑31 RA is observed.


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Applications and Data

 Recommended
Concentration
Sample
Flow Cytometry10 µL/106 cellsSee below


Flow Cytometry
Detection of IL‑31 RA in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Goat Anti-Human IL‑31 RA PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2769P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.


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Related Product & Information

BackgroundIL-31 RA
background_contentBackground:
IL-31 RA
The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356-745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co‑expression with OSM-R determines a cell's response to IL‑31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
    Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
    J Biol Chem, 2015;290(41):24747-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow



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