抗Alkaline Phosphatase抗体(Anti-Alkaline Phosphatase, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
Source	Monoclonal Mouse IgG1 Clone # B4-78
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
	Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline Phosphatase/ALPL and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	Alkaline Phosphatase/ALPL in Human Kidney. Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background	Alkaline Phosphatase/ALPL
background_content	Background: Alkaline Phosphatase/ALPL The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

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Citations

1. V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
   Authors: Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A
   PLoS ONE, 2015;10(3):e0118499.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy.
   Authors: Montespan F, Deschaseaux F, Sensebe L, Carosella E, Rouas-Freiss N
   J Immunol Res, 2014;2014(0):230346.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells.
   Authors: Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Fukuyama T, Wu L, Miwa T, Liu X, Wang X, Inoue T
   Bone, 2011;48(6):1417-26.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development.
   Authors: Choi SJ, Roodman GD, Feng JQ, Song IS, Amin K, Hart PS, Wright JT, Haruyama N, Hart TC
   Dev. Biol., 2009;325(1):129-37.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro.
   Authors: Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS
   Bone, 2009;45(2):309-20.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold.
   Authors: Valarmathi MT, Yost MJ, Goodwin RL, Potts JD
   Biomaterials, 2008;29(14):2203-16.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


7. Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice.
   Authors: Otsuru S, Tamai K, Yamazaki T, Yoshikawa H, Kaneda Y
   Biochem. Biophys. Res. Commun., 2007;354(2):453-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC

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