抗Fractalkine抗体(Anti-Fractalkine, Human, Mouse-Mono, PE antibody)

掲載日情報:2018/11/26 現在Webページ番号:33889

Fractalkineに対する抗体(Anti-Fractalkine, Human, Mouse-Mono, PE )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年06月19日 00時00分現在]

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納期 文献数
Anti-Fractalkine, Human, Mouse-Mono(51637.11), PE
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 9
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説明文
別名:ABCD-3
クローン:51637
Genbank No: 6376
Protein Accession No: P78423
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 PE
交差性 Human 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
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[在庫・価格 :2024年06月19日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Fractalkine, Human, Mouse-Mono(51637.11), PE

文献数: 9

説明文 別名:ABCD-3
クローン:51637
Genbank No: 6376
Protein Accession No: P78423
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Human 適用 FCM
標識 PE 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
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製品記事
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Product Details

Species ReactivityHuman
LabelPhycoerythrin
ImmunogenMouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain Gln25-Arg339 (Ser199Asn) (predicted)Accession # P78423
SourceMonoclonal Mouse IgG1 Clone # 51637
PurificationProtein A or G purified from ascites
SpecificityDetects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Intracellular Staining by Flow Cytometry10 µL/106 cellsSee below


Intracellular Staining by Flow Cytometry
Detection of CX3CL1/Fractalkine in NS0 Cell Line Transfected with His-tagged Human CX3CL1 by Flow Cytometry.
NS0 cell line transfected with His-tagged human CX3CL1 was stained with Mouse Anti-His Tag APC‑conjugated Monoclonal Antibody (Catalog # IC050A) and either (A) Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain PE‑conjugated Monoclonal Antibody (Catalog # IC365P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.


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Related Product & Information

BackgroundCX3CL1/Fractalkine
background_contentBackground:
CX3CL1/Fractalkine
CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Ralphalow and IL-7Ralphahigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine.
    Authors: Shin M, You S, Kang Y, Lee N, Yoo S, Park K, Kang K, Kim S, Mohanty S, Shaw A, Montgomery R, Hwang D, Kang I
    J Immunol, 2015;195(6):2861-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  2. Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells.
    Authors: Kassianos A, Wang X, Sampangi S, Afrin S, Wilkinson R, Healy H
    Kidney Int, 2015;87(6):1153-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  3. Functional adhesiveness of the CX3CL1 chemokine requires its aggregation. Role of the transmembrane domain.
    Authors: Hermand P, Pincet F, Carvalho S, Ansanay H, Trinquet E, Daoudi M, Combadiere C, Deterre P
    J. Biol. Chem., 2008;283(44):30225-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  4. CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis.
    Authors: Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD
    Blood, 2008;112(13):5026-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  5. Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes.
    Authors: Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A
    J. Immunol., 2007;178(12):8064-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

  6. Fractalkine mediates natural killer-dependent antitumor responses in vivo.
    Authors: Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C
    Cancer Res., 2003;63(21):7468-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow



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