抗Human IL-1 RI Phycoerythrin抗体(Anti-Human IL-1 RI Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-1 RI
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-1 RI in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of IL‑1 RI in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human IL‑1 RI PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB269P, filled histogram) or control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

IL-1 RI

background_content

Background: IL-1 RI Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex. An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

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Citations

1. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
   Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
   J Immunol, 2015;195(6):2818-28.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Differentiation of IL-17-producing effector and regulatory human T cells from lineage-committed naive precursors.
   Authors: Mercer F, Khaitan A, Kozhaya L, Aberg J, Unutmaz D
   J Immunol, 2014;193(3):1047-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1beta and TRIF/IRF3.
   Authors: Endo Y, Blinova K, Romantseva T, Golding H, Zaitseva M
   PLoS ONE, 2014;9(5):e98517.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Flow


4. Critical role of all-trans retinoic acid in stabilizing human natural regulatory T cells under inflammatory conditions.
   Authors: Lu L, Lan Q, Li Z, Zhou X, Gu J, Li Q, Wang J, Chen M, Liu Y, Shen Y, Brand D, Ryffel B, Horwitz D, Quismorio F, Liu Z, Li B, Olsen N, Zheng S
   Proc Natl Acad Sci U S A, 2014;111(33):E3432-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.
   Authors: Dalmas E, Venteclef N, Caer C, Poitou C, Cremer I, Aron-Wisnewsky J, Lacroix-Desmazes S, Bayry J, Kaveri S, Clement K, Andre S, Guerre-Millo M
   Diabetes, 2014;63(6):1966-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
   Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
   J Immunol, 2014;192(12):5776-88.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Regulating human Th17 cells via differential expression of IL-1 receptor.
   Authors: Lee WW, Kang SW, Choi J
   Blood, 2010;115(3):530-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
   Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
   J. Immunol., 2010;185(11):7047-56.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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