抗Human GITR/TNFRSF18 Phycoerythrin抗体(Anti-Human GITR/TNFRSF18 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18Gln26-Glu161 (Thr43Ala)Accession # Q9Y5U5
Source	Monoclonal Mouse IgG1 Clone # 110416
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse (rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of GITR/TNFRSF18 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL PHA for 24 hours were stained with Mouse Anti-Human GITR/TNFRSF18 PE-conjugated Mono­clonal Antibody (Catalog # FAB689P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

GITR/TNFRSF18

background_content

Background: GITR/TNFRSF18 GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up‑regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

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Citations

1. Therapeutic immune monitoring of CD4(+)CD25(+) T cells in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors
   Authors: Z Lu, N Xu, X Zhou, G Gao, L Li, J Huang, Y Li, Q Lu, B He, C Pan, X Liu
   Oncol Lett, 2017;14(2):1363-1372.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Statins increase the frequency of circulating CD4+ FOXP3+ regulatory T cells in healthy individuals.
   Authors: Rodriguez-Perea A, Montoya C, Olek S, Chougnet C, Velilla P
   J Immunol Res, 2015;2015(0):762506.
   Species: Human
   Sample Type: Whole Blood
   Application: Flow


3. Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation.
   Authors: Lages CS, Suffia I, Velilla PA, Huang B, Warshaw G, Hildeman DA, Belkaid Y, Chougnet C
   J. Immunol., 2008;181(3):1835-48.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Effects of the administration of high-dose interleukin-2 on immunoregulatory cell subsets in patients with advanced melanoma and renal cell cancer.
   Authors: van der Vliet HJ, Koon HB, Yue SC, Uzunparmak B, Seery V, Gavin MA, Rudensky AY, Atkins MB, Balk SP, Exley MA
   Clin. Cancer Res., 2007;13(7):2100-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Antigen-induced regulatory T cells in HBV chronically infected patients.
   Authors: Barboza L, Salmen S, Goncalves L, Colmenares M, Peterson D, Montes H, Cartagirone R, Gutierrez Mdel C, Berrueta L
   Virology, 2007;368(1):41-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
   Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
   J. Immunol., 2006;177(9):5811-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
   Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
   Blood, 2005;105(2):750-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Flow


8. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
   Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
   Blood, 2004;104(2):453-61.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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