抗FAP抗体(Anti-FAP, Sheep-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAPLeu26-Asp760Accession # Q12884
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human Fibroblast Activation Protein  alpha /FAP (Catalog # 3715-SE), see our available Western blot detection antibodies

Western Blot
	Detection of Human Fibroblast Activation Protein  alpha /FAP by Western Blot. Western blot shows lysates of WI‑38 human lung fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Fibroblast Activation Protein  alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibroblast Activation Protein  alpha /FAP at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Fibroblast Activation Protein  alpha /FAP in Human Squamous Cell Carcinoma. Fibroblast Activation Protein  alpha /FAP was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein  alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	Fibroblast Activation Protein  alpha /FAP in Human Basal Cell Carcinoma. Fibroblast Activation Protein  alpha /FAP was detected in immersion fixed paraffin-embedded sections of human basal cell carcinoma using Sheep Anti-Human Fibroblast Activation Protein  alpha /FAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3715) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Entrez Gene IDs	2191 (Human); 14089 (Mouse)
Background	Fibroblast Activation Protein alpha/FAP
background_content	Background: Fibroblast Activation Protein alpha/FAP FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.

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Citations

1. Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells
   Authors: MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields
   Nat Commun, 2018;9(1):948.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. A synthetic urinary probe-coated nanoparticles sensitive to fibroblast activation protein ? for solid tumor diagnosis
   Authors: X Feng, Q Wang, Y Liao, X Zhou, Y Wang, W Liu, G Zhang
   Int J Nanomedicine, 2017;12(0):5359-5372.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma
   Authors: A Lo, CP Li, EL Buza, R Blomberg, P Govindaraj, D Avery, J Monslow, M Hsiao, E Puré
   JCI Insight, 2017;2(19):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


4. Adrenergic-mediated increases in INHBA drive CAF phenotype and collagens
   Authors: AS Nagaraja, RL Dood, G Armaiz-Pen, Y Kang, SY Wu, JK Allen, NB Jennings, LS Mangala, S Pradeep, Y Lyons, M Haemmerle, KM Gharpure, NC Sadaoui, C Rodriguez-, C Ivan, Y Wang, K Baggerly, P Ram, G Lopez-Bere, J Liu, SC Mok, L Cohen, SK Lutgendorf, SW Cole, AK Sood
   JCI Insight, 2017;2(16):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Tumor-targeting efficacy of a BF211 prodrug through hydrolysis by fibroblast activation protein-?
   Authors: XP Chai, GL Sun, YF Fang, LH Hu, X Liu, XW Zhang
   Acta Pharmacol. Sin., 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice
   Authors: MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré
   J. Biol. Chem., 2016;291(15):8070-89.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: IP

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