抗CXCR-4抗体(Anti-CXCR-4, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma lineAccession # P61073
Source	Monoclonal Mouse IgG2A Clone # 12G5
Purification	Protein A or G purified from ascites
Specificity	Detects human CXCR4.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-reported	Nair, N. et al. (2016) Mucosal Immunol. 9: 68. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 0.3-1.2 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Flow Cytometry
	Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB170) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Neutralization
	Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutrali­zation by Human CXCR4 Antibody. Recombinant Human/ Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF‑1 alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCR4 Monoclonal Antibody (Catalog # MAB170). The ND50 is typically 0.3-1.2 µg/mL.

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Related Product & Information

Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Background	CXCR4
background_content	Background: CXCR4 CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.

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Citations

1. CXCR7 maintains osteosarcoma invasion after CXCR4 suppression in bone marrow microenvironment
   Authors: Y Han, C Wu, J Wang, N Liu
   Tumour Biol., 2017;39(5):1010428317701.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. ?20 IFITM2 differentially restricts X4 and R5 HIV-1
   Authors: WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang
   Proc. Natl. Acad. Sci. U.S.A., 2017;114(27):7112-7117.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4
   Authors: SC Mills, L Howell, A Beekman, L Stokes, A Mueller
   Cell. Signal., 2017;42(0):88-96.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1?
   Mol Cell Biol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut Ctrl


5. Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


6. I-bodies: human single domain antibodies that antagonize chemokine receptor CXCR4
   Authors: K Griffiths, O Dolezal, B Cao, SK Nilsson, HB See, KD Pfleger, M Roche, PR Gorry, A Pow, K Viduka, K Lim, BG Lu, DH Chang, TA Murray-Rus, M Kvansakul, MA Perugini, C Dogovski, M Doerflinge, Y Zhang, K Parisi, JL Casey, SD Nuttall, M Foley
   J Biol Chem, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


7. The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive Therapy.
   Authors: Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann I, Levine B, June C, Zhang L, Wang E, Marincola F, Bedognetti D, Powell D, Tanyi J, Feldman M, Kandalaft L, Coukos G
   Clin Cancer Res, 2015;21(12):2840-50.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
   Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
   PLoS ONE, 2015;10(3):e0120069.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Enhanced Chemokine Receptor Recycling and Impaired S1P1 Expression Promote Leukemic Cell Infiltration of Lymph Nodes in Chronic Lymphocytic Leukemia.
   Authors: Patrussi L, Capitani N, Martini V, Pizzi M, Trimarco V, Frezzato F, Marino F, Semenzato G, Trentin L, Baldari C
   Cancer Res, 2015;75(19):4153-63.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. An isoform of C/EBPbeta, LIP, regulates expression of the chemokine receptor CXCR4 and modulates breast cancer cell migration.
    Authors: Park B, Kook S, Lee S, Jeong J, Brufsky A, Lee B
    J Biol Chem, 2013;288(40):28656-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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