抗Human PDGF R beta Fluorescein抗体(Anti-Human PDGF R beta Fluorescein antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	Human skin fibroblast membrane extracts
Source	Monoclonal Mouse IgG1 Clone # PR7212
Purification	Protein A or G purified from ascites
Specificity	Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta -subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF receptor alpha ‑subunit. Detects the PDGF receptor beta -subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1263F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

PDGF R beta

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Background: PDGF R beta PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immuno‑globulinlike domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist (4).

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Citations

1. Somatic GNAQ Mutation is Enriched in Brain Endothelial Cells in�Sturge-Weber Syndrome
   Authors: Lan Huang
   Pediatr. Neurol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
   Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
   Stem Cells, 2015;33(1):133-45.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
   Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
   Exp. Cell Res., 2012;318(4):371-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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