抗Myocardin抗体(Anti-Myocardin, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Myocardin Met97-Leu290Accession # Q8IZQ8
Source	Monoclonal Mouse IgG2B Clone # 355521
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human and mouse Myocardin in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below

Western Blot

Detection of Human Myocardin by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Entrez Gene IDs	93649 (Human); 214384 (Mouse); 246297 (Rat)
Background	Myocardin
background_content	Background: Myocardin Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.

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Citations

1. Downregulation of Insulin Receptor Substrate-1 During Hyperglycemia Induces Vascular Smooth Muscle Cell Dedifferentiation
   Authors: Gang Xi
   J. Biol. Chem, 2016;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation
   J Am Heart Assoc, 2016;5(12):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
   Authors: Yao Y, Chen Z, Norris E, Strickland S
   Nat Commun, 2014;5(0):3413.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Co-activation of nuclear factor-kappaB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts.
   Authors: Madonna R, Geng Y, Bolli R, Rokosh G, Ferdinandy P, Patterson C, De Caterina R
   J Biol Chem, 2014;289(28):19585-98.
   Species: Canine
   Sample Type: Cell Lysates
   Application: WB


5. Akt1 mediates alpha-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor.
   Authors: Abdalla M, Goc A, Segar L, Somanath P
   J Biol Chem, 2013;288(46):33483-93.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia.
   Authors: Madonna R, Taylor D, Geng Y, De Caterina R, Shelat H, Perin E, Willerson J
   Circ Res, 2013;113(7):902-14.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification.
   Authors: Lu, Tzong-Sh, Lim, Kenneth, Molostvov, Guerman, Yang, Yun-Chun, Yiao, Szu-Yu, Zehnder, Daniel, Hsiao, Li-Li
   Cardiovasc Res, 2012;96(3):524-32.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling.
   Authors: Pfisterer, Larissa, Feldner, Anja, Hecker, Markus, Korff, Thomas
   Cardiovasc Res, 2011;96(1):120-9.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4.
   Authors: Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A
   J. Biol. Chem., 2011;286(32):28097-110.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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