抗SMAD7抗体(Anti-SMAD7, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human Smad7Gly320-Ser398Accession # O15105
Source	Monoclonal Mouse IgG2B Clone # 293039
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human, mouse, and rat Smad7.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	See below

Immunohistochemistry
	Smad7 in Human Liver Cancer Tissue. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot
	Detection of Human/Mouse/Rat Smad7 by Western Blot. Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Immunohistochemistry
	Smad7 in Human Liver. Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human Smad7 by Simple WesternTM. Simple Western lane view shows lysates of Sf 21 S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Mothers Against DPP Homolog 7
Background	Smad7
background_content	Background: Smad7 Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.

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Citations

1. Smooth muscle cells differentiated from human mesenchymal stem cells regulated by microRNA (miR)-503 and miR-222-5p are suitable for vascular tissue engineering
   Authors: W Gu, X Hong, A Le Bras, WN Nowak, S Issa Bhalo, J Deng, Y Xie, Y Hu, XZ Ruan, Q Xu
   J. Biol. Chem., 2018;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. High SMAD7 and p-SMAD2,3 expression is associated with Environmental Enteropathy in children
   Authors: S Syed, V Dinallo, NT Iqbal, L Di Iorio, D Di Fusco, S Guleria, BC Amadi, K Sadiq, C Moskaluk, SA Ali, P Kelly, G Monteleone
   PLoS Negl Trop Dis, 2018;12(2):e0006224.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


3. Smad7 knockdown activates protein kinase RNA-associated eIF2? pathway leading to colon cancer cell death
   Authors: V De Simone, G Bevivino, S Sedda, R Izzo, F Laudisi, V Dinallo, E Franzè, A Colantoni, A Ortenzi, S Salvatori, P Rossi, GS Sica, MC Fantini, C Stolfi, G Monteleone
   Cell Death Dis, 2017;8(3):e2681.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-? signaling in and promote progression of hepatocellular carcinoma
   Authors: H Sun, Z Peng, H Tang, D Xie, Z Jia, L Zhong, S Zhao, Z Ma, Y Gao, L Zeng, R Luo, K Xie
   Oncogene, 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. Hepatocyte-specific Smad7 deletion accelerates DEN-induced HCC via activation of STAT3 signaling in mice
   Authors: T Feng, J Dzieran, X Yuan, A Dropmann, T Maass, A Teufel, S Marhenke, T Gaiser, F Rückert, I Kleiter, S Kanzler, MP Ebert, A Vogel, P Ten Dijke, S Dooley, NM Meindl-Bei
   Oncogenesis, 2017;6(1):e294.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells
   Authors: Boris Hinz
   Nat Mater, 2016;0(0):.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


7. TGF-Beta Induces Endometriotic Progression via a Non-Canonical, KLF11 Mediated Mechanism
   Endocrinology, 2016;0(0):en20161194.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis
   Nat Commun, 2016;7(0):13498.
   Application: WB


9. Downregulation of the TGF-? pseudoreceptor BAMBI in non-small cell lung cancer enhances TGF-? signaling and invasion
   Cancer Res, 2016;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. The TGF?-SMAD3 pathway inhibits IL-1? induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration
    J Exp Clin Cancer Res, 2016;35(1):122.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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