抗Axl抗体(Anti-Axl, Phosphorylated, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Phosphopeptide containing human Axl Y779 site
Source	Polyclonal Rabbit IgG
Purification	Antigen and protein A Affinity-purified
Specificity	Detects human and mouse Axl when phosphorylated at Y779.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Immunohistochemistry
	Phospho-Axl (Y779) in Human Stomach Cancer Tissue. Axl phosphorylated at site Y779 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot
	Detection of Human Phospho-Axl (Y779) by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line untreated (-) or treated (+) with WI38 human lung cell line conditioned media (WI38 CM) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Human Phospho‑Axl (Y779) Antigen Affinity‑purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho‑Axl (Y779) at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Phospho-Axl (Y779) in A172 Human Cell Line. Axl phosphorylated at Y779 (panels B, D) and total Axl (panels A, C) were assessed in immersion fixed A172 human glioblastoma cells incubated with (panels C, D) or without (panels A, B) pervanadate. Phospho-Axl was detected using Rabbit Anti-Human Phospho-Axl (Y779) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2228) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, panels B, D); Catalog # NL004) and counterstained using DAPI (blue). Total Axl was detected using Goat Anti-Human Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF154). Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, panels A, C); Catalog # NL003). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Axl

background_content

Background: Axl Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The human Axl cDNA encodes an 887 amino acid (aa) precursor that includes an 18 aa signal sequence, a 426 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of human and mouse Axl share 81% aa sequence identity. A short alternately spliced form of human Axl is distinguished by a 9 aa deletion in the extracellular juxtamembrane region. These receptors bind the vitamin K‑dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

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Citations

1. DAPK and CIP2A are involved in GAS6/AXL-mediated Schwann cell proliferation in a rat model of bilateral cavernous nerve injury
   Authors: YL Chen, YT Tsai, TT Chao, YN Wu, MC Chen, YH Lin, CH Liao, SP Chou, HS Chiang
   Oncotarget, 2018;9(5):6402-6415.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


2. BET inhibition overcomes receptor tyrosine kinase-mediated cetuximab resistance in HNSCC
   Authors: B Leonard, TM Brand, RA O'Keefe, E Lee, Y Zang, JD Kemmer, H Li, JR Grandis, NE Bhola
   Cancer Res., 2018;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Gas6 derived from cancer-associated fibroblasts promotes migration of Axl-expressing lung cancer cells during chemotherapy
   Authors: R Kanzaki, H Naito, K Kise, K Takara, D Eino, M Minami, Y Shintani, S Funaki, T Kawamura, T Kimura, M Okumura, N Takakura
   Sci Rep, 2017;7(1):10613.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
   Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
   Br. J. Cancer, 2017;117(12):1787-1797.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL
   Authors: K Iida, R Sakai, S Yokoyama, N Kobayashi, S Togo, HY Yoshikawa, A Rawangkan, K Namiki, M Suganuma
   Sci Rep, 2017;7(1):17770.
   Species: Human
   Sample Type: Whole Cells
   Application: WB


6. Tropomyosin isoform Tpm2.1 regulates collective and amoeboid cell migration and cell aggregation in breast epithelial cells
   Authors: H Shin, D Kim, DM Helfman
   Oncotarget, 2017;8(56):95192-95205.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Receptor tyrosine kinase Axl is required for resistance of leukemic cells to FLT3-targeted therapy in acute myeloid leukemia.
   Authors: Park I, Mundy-Bosse B, Whitman S, Zhang X, Warner S, Bearss D, Blum W, Marcucci G, Caligiuri M
   Leukemia, 2015;29(12):2382-9.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis.
   J. Exp. Med., 2012;209(11):2033-47.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. The novel receptor tyrosine kinase Axl is constitutively active in B-cell chronic lymphocytic leukemia and acts as a docking site of nonreceptor kinases: implications for therapy.
   Authors: Ghosh AK, Secreto C, Boysen J, Sassoon T, Shanafelt TD, Mukhopadhyay D, Kay NE
   Blood, 2011;117(6):1928-37.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


10. Growth arrest-specific protein 6 deficiency impairs liver tissue repair after acute toxic hepatitis in mice.
    Authors: Lafdil F, Chobert MN, Deveaux V, Zafrani ES, Mavier P, Nakano T, Laperche Y, Brouillet A
    J. Hepatol., 2009;51(1):55-66.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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