抗Flt-4抗体(Anti-Flt-4, Human, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human VEGF R3/Flt-4Tyr25-Ile776Accession # P35916
Source	Monoclonal Mouse IgG1 Clone # 54733
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VEGF R3/Flt-4 in direct ELISAs.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of VEGF R3/Flt‑4 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R3/Flt‑4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3492P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Background

VEGF R3/Flt-4

background_content

Background: VEGF R3/Flt-4 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGF R3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain, and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.

目次に戻る

Citations

1. BRAF Inhibition Stimulates Melanoma-Associated Macrophages to Drive Tumor Growth.
   Authors: Wang T, Xiao M, Ge Y, Krepler C, Belser E, Lopez-Coral A, Xu X, Zhang G, Azuma R, Liu Q, Liu R, Li L, Amaravadi R, Xu W, Karakousis G, Gangadhar T, Schuchter L, Lieu M, Khare S, Halloran M, Herlyn M, Kaufman R
   Clin Cancer Res, 2015;21(7):1652-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
   Authors: Fiorio Pla, Alessand, Brossa, Alessia, Bernardini, Michela, Genova, Tullio, Grolez, Guillaum, Villers, Arnaud, Leroy, Xavier, Prevarskaya, Natalia, Gkika, Dimitra, Bussolati, Benedett
   BMC Cancer, 2014;14(0):939.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Expression of VEGF receptors on endothelial cells in mouse skeletal muscle.
   Authors: Imoukhuede, Princess, Popel, Aleksand
   PLoS ONE, 2012;7(9):e44791.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation.
   Authors: Fernandez L, Rodriguez S, Huang H, Chora A, Fernandes J, Mumaw C, Cruz E, Pollok K, Cristina F, Price JE, Ferkowicz MJ, Scadden DT, Clauss M, Cardoso AA, Carlesso N
   Exp. Hematol., 2008;36(5):545-558.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Highly efficient and feeder-free production of subculturable vascular endothelial cells from primate embryonic stem cells.
   Authors: Saeki K, Yogiashi Y, Nakahara M, Nakamura N, Matsuyama S, Koyanagi A, Yagita H, Koyanagi M, Kondo Y, Yuo A
   J. Cell. Physiol., 2008;217(1):261-80.
   Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
   Sample Type: Whole Cells
   Application: Flow


6. Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
   Authors: Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J
   J. Clin. Invest., 2007;117(11):3369-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
   Authors: Ball SG, Shuttleworth CA, Kielty CM
   J. Cell Biol., 2007;177(3):489-500.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

目次に戻る