抗TREM-1抗体(Anti-TREM-1, Mouse, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse TREM-1Ala21-Ser202Accession # Q9JKE2
Source	Monoclonal Rat IgG2A Clone # 174031
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry

Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody(Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB1187P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

TREM-1

background_content

Background: TREM-1 TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

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Citations

1. HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
   Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
   Sci Rep, 2017;7(0):42028.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. TREM-1low is a novel characteristic for tumor-associated macrophages in lung cancer
   Oncotarget, 2016;7(26):40508-40517.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
   Authors: Yuan Z, Syed M, Panchal D, Joo M, Colonna M, Brantly M, Sadikot R
   J Biol Chem, 2014;289(21):15118-29.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Stimulation of the molecule 4-1BB enhances host defense against Listeria monocytogenes infection in mice by inducing rapid infiltration and activation of neutrophils and monocytes.
   Authors: Lee SC, Ju SA, Sung BH, Heo SK, Cho HR, Lee EA, Kim JD, Lee IH, Park SM, Nguyen QT, Suh JH, Kim BS
   Infect. Immun., 2009;77(5):2168-76.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. TREM-1 promotes survival during septic shock in mice.
   Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
   Eur. J. Immunol., 2007;37(2):456-66.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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