抗Human Fc gamma RI/CD64 Phycoerythrin 二次抗体(Anti-Human Fc gamma RI/CD64 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Fc gamma  RIGln16-Pro288Accession # P12314.2
Source	Monoclonal Mouse IgG1 Clone # 276426
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Fc gamma RI in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) Fc gamma  RI, rmFc gamma  RII, recombinant human (rh) Fc gamma  RIIA, rhFc gamma  RIIB, or rhFc gamma  RIIIB.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of Fc gamma  RI/CD64 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma  RI/CD64 PE‑conjugated Monoclonal Antibody (Catalog # FAB12571P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

Fc gamma RI/CD64

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Background: Fc gamma RI/CD64 Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cell-mediated cytotoxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8 - 10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6 - 10-7 M), only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains, or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis. Over amino acids 16-288, human CD64 shares 72% amino acid sequence identity with mouse CD64.

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Citations

1. Chemically synthesized molecules with the targeting and effector functions of antibodies.
   Authors: McEnaney P, Fitzgerald K, Zhang A, Douglass E, Shan W, Balog A, Kolesnikova M, Spiegel D
   J Am Chem Soc, 2014;136(52):18034-43.
   Species: N/A
   Sample Type: Protein
   Application: Binding Assay


2. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
   Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
   J. Thromb. Haemost., 2011;9(5):1056-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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