抗OX40抗体(Anti-OX40, Sheep-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：33083
OX40に対する抗体(Anti-OX40, Sheep-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2025年07月01日 18時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
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文献数 | ||
|---|---|---|---|---|---|
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Anti-OX40, Sheep-Poly <Anti-TNFRSF4> |
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0 | |||
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Anti-Human OX40/TNFRSF4 Affinity Purified Polyclonal Ab |
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0 | |||
[在庫・価格 ：2025年07月01日 18時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-OX40, Sheep-Poly <Anti-TNFRSF4>
文献数： 0
- 商品コード：AF3388
- メーカー：RSD
- 包装：100μg
- 価格：¥83,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human OX40/TNFRSF4 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF3388-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥25,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human OX40Leu29-Ala216Accession # P43489 |
| Source | Polyclonal Sheep IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human OX40/TNFRSF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse OX40 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.5 µg/mL | See below |
| Flow Cytometry | 0.25 µg/106 cells | See below |
| Immunohistochemistry | 3-15 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Western Blot | |
|---|---|
 | Detection of Human OX40/TNFRSF4 by Western Blot. Western blot shows lysates of human T regulatory cells (Tregs) and human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 1 μg/mL PHA for 5 days. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human OX40/TNFRSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3388) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for OX40/TNFRSF4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Flow Cytometry | |
 | Detection of OX40/TNFRSF4 in Human T Cells by Flow Cytometry. Human T cells were treated for 3‑5 days with 1 μg/mL PHA then stained with Sheep Anti-Human OX40/TNFRSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3388, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010). |
| Immunohistochemistry | |
 | OX40/TNFRSF4 in Human Tonsil. OX40/TNFRSF4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Sheep Anti-Human OX40/TNFRSF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3388) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Related Product & Information
| Background | OX40/TNFRSF4 |
|---|---|
| background_content | Background: OX40/TNFRSF4 OX40 (CD134; TNFRSF4) is a T cell costimulatory molecule of the TNF receptor superfamily that coordinates with other membrane-bound costimulators such as CD28, CD40, CD30, CD27 and 4-1BB (1‑3). OX40 is expressed on naïve CD4+ T cells only after engagement of the TCR by antigen presenting cells (APC; dendritic and B cells), and costimulation by CD40/CD40 ligand and CD28/B7. It is maximal at 2‑5 days post activation, or 4 hours post reactivation of memory T cells (3‑6). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that has four TNFR-Cys repeats and an O-glycosylated hinge region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (3). The ECD of human OX40 shows 71%, 68%, 67%, 64% and 64% aa identity with feline, canine, rabbit, mouse and rat OX40 ECD, respectively. Engagement of OX40 on activated CD4+ T cells by OX40 ligand on activated dendritic cells promotes T cell survival and proliferation, prolongs the immune response, and enhances the number of cells making the transition from effector to memory T cells (1‑6). OX40 signal transduction includes binding TNF receptor-associated factors (TRAFs), and activating NF kappa B and PI3 kinase to enhance expression of cytokines, antiapoptotic Bcl-2 family members, survivin and the chemokine receptor CXCR5 (5‑8). CXCR5 promotes T cell migration to germinal centers to deliver B cell help (5). Studies using knockout or transgenic mice, and agonistic or blocking antibodies, show that OX40/OX40L interaction is critical for establishing or reactivating memory T cells and breaking immune tolerance (9). Blockade of OX40 engagement is efficacious in animal models of allergic airway inflammation, graft-versus-host disease and autoimmune disease (10‑14). |
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