抗LIN-28抗体(Anti-LIN-28, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human LIN-28AMet1-Asn209Accession # Q9H9Z2
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human LIN-28A in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human LIN‑28A by Western Blot. Western blot shows lysates of JAR human choriocarcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LIN‑28A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	LIN‑28A in BG01V Human Stem Cells. LIN‑28A was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	LIN‑28A in D3 Mouse Stem Cells. LIN‑28A was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Long Name	RNA-binding Protein LIN-28
Background	LIN-28A
background_content	Background: LIN-28A Human LIN-28 (Protein lin-28 homolog A; CSDD1, LIN28 and ZCCHC1)  is a 30 kDa (209 amino acids) cytoplasmic RNA-binding protein with an N-terminal cold shock domain and two C-terminal CCHC zinc finger domains. It is expressed by various undifferentiated embryonic cell types and is also present in adult cardiac and skeletal muscle. Expression of LIN-28 has been shown to be regulated by micro-RNA. Human LIN-28 shares 98% and 97% amino acid sequence homology with rat and mouse LIN-28, respectively.

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Citations

1. Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX
   Authors: S Ramaswamy, N Tonnu, T Menon, BM Lewis, KT Green, D Wampler, PE Monahan, IM Verma
   Cell Rep, 2018;23(5):1565-1580.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture
   Authors: AR Helsel, MJ Oatley, JM Oatley
   Stem Cell Reports, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


3. Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate
   Authors: T Shibata, Y Fujita, H Ohno, Y Suzuki, K Hayashi, KR Komatsu, S Kawasaki, K Hidaka, S Yonehara, H Sugiyama, M Endo, H Saito
   Nat Commun, 2017;8(1):540.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Embryonic lethality and defective male germ cell development in mice lacking UTF1
   Authors: SD Kasowitz, M Luo, J Ma, NA Leu, PJ Wang
   Sci Rep, 2017;7(1):17259.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture.
   Authors: Makela J, Toppari J, Rivero-Muller A, Ventela S
   PLoS ONE, 2014;9(3):e90088.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach.
   Authors: Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J
   PLoS ONE, 2010;5(12):e14397.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
   Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
   Cell, 2007;131(5):861-72.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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