抗TIM-3抗体(Anti-TIM-3, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Mouse myeloma cell line NS0-derived recombinant human TIM‑3Ser22-Arg200Accession # Q8TDQ0.2
Source	Monoclonal Rat IgG2A Clone # 344823
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of TIM‑3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Rat Anti-Human TIM‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB2365P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

TIM-3

background_content

Background: TIM-3 TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2, is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region (1, 2). Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (3). An alternatively spliced isoform is truncated within the mucin-like stalk. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up‑regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) (3-10). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity (11). TIM-3 ligation attenuates CD8+ and Th1 cell responses (11-13) and promotes the activity of Treg and myeloid derived suppressor cells (8, 11, 13, 14). In addition, dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens (4). It also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA (7). TIM-3 interactions with Galectin-9 can alternatively trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity (10).

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Citations

1. Phase I trial of plerixafor combined with decitabine in newly diagnosed older patients with acute myeloid leukemia
   Authors: GJ Roboz, EK Ritchie, Y Dault, L Lam, DC Marshall, NM Cruz, HC Hsu, DC Hassane, PJ Christos, C Ippoliti, JM Scandura, ML Guzman
   Haematologica, 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells
   Authors: C Stecher, C Battin, J Leitner, M Zettl, K Grabmeier-, C Höller, GJ Zlabinger, P Steinberge
   Front Immunol, 2017;8(0):572.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. TCR-ligand dissociation rate is a robust and stable biomarker of CD8+ T cell potency
   Authors: M Allard, B Couturaud, L Carretero-, MN Duong, J Schmidt, GC Monnot, P Romero, DE Speiser, M Hebeisen, N Rufer
   JCI Insight, 2017;2(14):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Synergistic effect of IL-12 and IL-18 induces TIM3 regulation of ?? T cell function and decreases the risk of clinical malaria in children living in Papua New Guinea
   Authors: L Schofield, LJ Ioannidis, S Karl, LJ Robinson, QY Tan, DP Poole, I Betuela, DL Hill, PM Siba, DS Hansen, I Mueller, EM Eriksson
   BMC Med, 2017;15(1):114.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE
   Authors: D Zhao, M Guo, B Liu, Q Lin, T Xie, Q Zhang, X Jia, Q Shu, X Liang, L Gao, C Ma
   J. Leukoc. Biol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. BCG vaccination induces HIV target cell activation in HIV-exposed infants in a randomized trial
   Authors: MA Gasper, AC Hesseling, I Mohar, L Myer, T Azenkot, JS Passmore, W Hanekom, MF Cotton, IN Crispe, DL Sodora, HB Jaspan
   JCI Insight, 2017;2(7):e91963.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
   Authors: JA Schwartz, KL Clayton, S Mujib, H Zhang, AK Rahman, J Liu, FY Yue, E Benko, C Kovacs, MA Ostrowski
   J. Immunol, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients
   Sci Rep, 2016;6(0):25567.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo
   J Clin Invest, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
    J Clin Invest, 2016;126(8):3130-44.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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