抗Human FoxJ1抗体(Anti-Human FoxJ1 antibody)

掲載日情報:2018/11/26 現在Webページ番号:32720

Human FoxJ1に対する抗体(Anti-Human FoxJ1 )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2024年05月05日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-Human FoxJ1 Affinity Purified Polyclonal Ab (100 UG)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 4
説明文
別名:FKHL13
Genbank No: 2302
Protein Accession No: Q92949
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 IC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 肺オルガノイド培養関連製品(Products for Lung Organoids)
関連記事
Anti-Human FoxJ1 Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:FKHL13
Genbank No: 2302
Protein Accession No: Q92949
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 IC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 肺オルガノイド培養関連製品(Products for Lung Organoids)
関連記事

[在庫・価格 :2024年05月05日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Human FoxJ1 Affinity Purified Polyclonal Ab (100 UG)

文献数: 4

説明文 別名:FKHL13
Genbank No: 2302
Protein Accession No: Q92949
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 IC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 肺オルガノイド培養関連製品(Products for Lung Organoids)
関連記事

Anti-Human FoxJ1 Affinity Purified Polyclonal Ab

文献数: 2

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:FKHL13
Genbank No: 2302
Protein Accession No: Q92949
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 IC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 肺オルガノイド培養関連製品(Products for Lung Organoids)
関連記事



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenE. coli-derived recombinant human FoxJ1 Lys306-Leu421Accession # Q92949
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human FoxJ1 in direct ELISAs and Western blots.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLSee below
Simple Western10 µg/mLSee below
Immunocytochemistry5-15 µg/mLSee below


Western Blot
Detection of Human FoxJ1 by Western Blot.
Western blot shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxJ1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
FoxJ1 in HEK293 Human Cell Line.
FoxJ1 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
FoxJ1 in Early Proximal Lung Progenitors.
FoxJ1 was detected in immersion fixed BGO1V human embryonic stem cells differentiated to early proximal lung progenitors using Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western
Detection of Human FoxJ1 by Simple WesternTM.
Simple Western lane view shows lysates of Capan‑1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxJ1 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.


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Related Product & Information

Long NameForkhead Box J1
BackgroundFoxJ1
background_contentBackground:
FoxJ1
FoxJ1 (Forkhead box protein J1; also HFH4) is a 55-65 kDa, class 1 member of the HNF-3/forkhead gene family of transcription factors. Its observed MW is higher than its predicted MW (45 kDa), suggesting anomalous migration on SDS-PAGE. FoxJ1 promotes the expression of cilia in respiratory, oviduct and choroid plexus epithelium, and maintains T cell tolerance to self-antigens. Human FoxJ1 is 421 amino acids (aa) in length, and contains one forkhead DNA binding domain (aa's 120‑210). The presence of basic residues in the forkhead domain makes FoxJ1 a class 1 Fox protein. There is potential splice variant that contains a 25 aa substation for aa 262-287. Over aa 306-421, human FoxJ1 shares 88% aa with mouse FoxJ1.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Gefitinib, an EGFR Tyrosine Kinase inhibitor, Prevents Smoke-Mediated Ciliated Airway Epithelial Cell Loss and Promotes Their Recovery
    PLoS ONE, 2016;11(8):e0160216.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded

  2. Hyaluronan fragments/CD44 mediate oxidative stress-induced MUC5B up-regulation in airway epithelium.
    Authors: Casalino-Matsuda SM, Monzon ME, Day AJ, Forteza RM
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):277-85.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded



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