抗SOX1抗体(Anti-SOX1, Goat-Poly antibody)

掲載日情報:2018/11/26 現在Webページ番号:32655

SOX1に対する抗体(Anti-SOX1, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

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[在庫・価格 :2024年04月20日 12時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-SOX1, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 44
説明文
別名:SRY (sex determining region Y)-box 1
Genbank No: 6656
Protein Accession No: NP_005977
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 IC,IHC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 抗幹細胞マーカー抗体/抗神経幹細胞マーカー抗体
StemXVivo Endoderm / Ectoderm / Mesoderm Kit
関連記事
Anti-Human SOX1 Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 13
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:SRY (sex determining region Y)-box 1
Genbank No: 6656
Protein Accession No: NP_005977
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 IC,IHC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事

[在庫・価格 :2024年04月20日 12時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-SOX1, Goat-Poly

文献数: 44

説明文 別名:SRY (sex determining region Y)-box 1
Genbank No: 6656
Protein Accession No: NP_005977
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 IC,IHC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 抗幹細胞マーカー抗体/抗神経幹細胞マーカー抗体
StemXVivo Endoderm / Ectoderm / Mesoderm Kit
関連記事

Anti-Human SOX1 Affinity Purified Polyclonal Ab

文献数: 13

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:SRY (sex determining region Y)-box 1
Genbank No: 6656
Protein Accession No: NP_005977
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 IC,IHC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

Species ReactivityHuman, Mouse, Rat
LabelUnconjugated
ImmunogenE. coli-derived recombinant human SOX1Asn242-Gly379 (Leu276Ile)Accession # NP_005977
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human SOX1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) SOX2 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLSee below
Simple Western10 µg/mLSee below
Immunocytochemistry5-15 µg/mLSee below


Western Blot
Detection of Human SOX1 by Western Blot.
Western blot shows lysates of undifferentiated iBJ6 human iPS cells and iBJ6 human iPS cells differentiated into neuroprogenitor cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX1 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
SOX1 in ectoderm differentuiated BG01V Human Embryonic Stem Cells.
SOX1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neural progenitor cells using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX1 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunocytochemistry
SOX1 in differentiated NTera‑2 Human Cell Line.
SOX1 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line differentiated with retinoic acid using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green, upper panel; Catalog # NL003) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
SOX1 in Rat Cortical Stem Cells.
SOX1 was detected in immersion fixed rat cortical stem cells (Catalog # NSC001) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
SOX1 in Mouse Cortical Stem Cells.
SOX1 was detected in immersion fixed mouse cortical stem cells (Catalog # NSC002) using Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
Detection of Human SOX1 by Simple WesternTM.
Simple Western lane view shows lysates of undifferentiated iBJ6 human iPS cells andiBJ6 human iPS cells differentiated into neuroprogenitor cells, loaded at 0.2 mg/mL. A specific band was detected for SOX1 at approximately 50 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SOX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3369) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.


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Related Product & Information

Long NameSRY-related HMG-box 1
BackgroundSOX1
background_contentBackground:
SOX1
SOX1 is a 39 kDa transcription factor that belongs to the SOXB1 subgroup. Within the developing CNS, SOX1 maintains neural cells in an undifferentiated state and has been used as a marker for neural stem cells. Human and mouse SOX1 share 97% amino acid sequence identity.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. TFAP2C regulates transcription in human naive pluripotency by opening enhancers
    Authors: WA Pastor, W Liu, D Chen, J Ho, R Kim, TJ Hunt, A Lukianchik, X Liu, JM Polo, SE Jacobsen, AT Clark
    Nat. Cell Biol., 2018;20(5):553-564.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

  2. Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells
    Authors: M Zhang, J Ngo, F Pirozzi, YP Sun, A Wynshaw-Bo
    Stem Cell Res Ther, 2018;9(1):67.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  3. Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease
    Authors: L Marrone, C Bus, D Schöndorf, JC Fitzgerald, M Kübler, B Schmid, P Reinhardt, L Reinhardt, M Deleidi, T Levin, A Meixner, B Klink, M Glatza, CJ Gloeckner, T Gasser, J Sternecker
    PLoS ONE, 2018;13(3):e0192497.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  4. Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells
    Authors: JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim
    Exp. Mol. Med., 2018;50(4):24.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  5. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018;22(4):543-558.e12.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

  6. Modeling the Mutational and Phenotypic Landscapes of Pelizaeus-Merzbacher Disease with Human iPSC-Derived Oligodendrocytes
    Authors: ZS Nevin, DC Factor, RT Karl, P Douvaras, J Laukka, MS Windrem, SA Goldman, V Fossati, GM Hobson, PJ Tesar
    Am. J. Hum. Genet., 2017;100(4):617-634.
    Application: ICC

  7. Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells
    Authors: P Walsh, V Truong, C Hill, ND Stoflet, J Baden, WC Low, SA Keirstead, JR Dutton, AM Parr
    Cell Transplant, 2017;26(12):1890-1902.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  8. Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells
    Authors: Y Li, R Wang, N Qiao, G Peng, K Zhang, K Tang, JJ Han, N Jing
    J. Biol. Chem., 2017;292(48):19590-19604.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  9. A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model
    Authors: X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky
    PLoS ONE, 2017;12(4):e0174074.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  10. Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state
    Authors: Rui Gao
    Biomaterials, 2017;119(0):53-67.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC



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