抗HSP90抗体(Anti-HSP90, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human HSP90 beta Met1-Asp724Accession # P08238
Source	Monoclonal Mouse IgG2B Clone # 341320
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human, mouse and rat HSP90 in direct ELISAs and Western blots. Reacts with both recombinant human HSP90 alpha and HSP90 beta isoforms in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below

Western Blot
	Detection of Human/Mouse/Rat HSP90 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HSP90 alpha and HSP90 beta (5 ng/lane) were included. A specific band for HSP90 was detected at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Simple Western
	Detection of Human and Rat HSP90 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP90 at approximately 95 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Long Name	Heat Shock Protein 90
Background	HSP90
background_content	Background: HSP90 HSP90 is a composite name for a large group of Heat Shock Proteins whose molecular weights average 90 kDa. HSPs functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP90 is a central component in a number of basic cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved and accounts for 1‑2% of the total cellular protein. Recently introduced, standardized nomenclature has divided the 17 identified HSP90 genes into three related and one unrelated classes, HSP90AA, HSP90AB, HSP90BB, and TRAP, respectively. Eukaryotic cells have two principal isoforms of HSP90. The antibody described here reacts with the 724 amino acid constitutively expressed HSP90AB1-isoform 1 (also known as HSP90 beta, HSP90B, HSPCB, HSPC2, and HSP89 beta ) and also with the 737 amino acid inducible form, HSP90AA1 (also known as HSP90 alpha, HSP90A, HSPC1, HSPCA, HSP89 alpha and LAP2). HSP90AB1-1 and HSP90AA share 90% identity.

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Citations

1. Nuclear pore complex-mediated modulation of TCR signaling is required for na�ve CD4+ T cell homeostasis
   Authors: J Borlido, S Sakuma, M Raices, F Carrette, R Tinoco, LM Bradley, MA D'Angelo
   Nat. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Nuclear Pores Regulate Muscle Development and Maintenance by Assembling a Localized Mef2C Complex
   Authors: M Raices, L Bukata, S Sakuma, J Borlido, LS Hernandez, DO Hart, MA D'Angelo
   Dev. Cell, 2017;41(5):540-554.e7.
   Species: Zebrafish
   Sample Type: Protein
   Application: WB


3. Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori
   Authors: Guangxin Li
   Am J Transl Res, 2016;8(10):4184-4194.
   Species: Rat
   Sample Type: Whole Cells
   Application: WB


4. Mutation in integrin-linked kinase (ILK(R211A)) and heat-shock protein 70 comprise a broadly cardioprotective complex.
   Authors: Traister, Alexandr, Walsh, Mark, Aafaqi, Shabana, Lu, Minglian, Dai, Xiaojing, Henkleman, Mark R, Momen, Abdul, Zhou, Yu-Quing, Husain, Mansoor, Arab, Sara, Piran, Sara, Hannigan, Gregory, Coles, John G
   PLoS ONE, 2013;8(11):e77331.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Glucocorticoids enhance CD163 expression in placental Hofbauer cells.
   Authors: Tang Z, Niven-Fairchild T, Tadesse S, Norwitz E, Buhimschi C, Buhimschi I, Guller S
   Endocrinology, 2013;154(1):471-82.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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