抗ULBP-2抗体(Anti-ULBP-2, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	BaF3 mouse pro-B cell line transfected with human ULBP-2Accession # Q9BZM5
Source	Monoclonal Mouse IgG2A Clone # 165903
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human ULBP-2 and human RAET1L/ULBP-6 in direct ELISA and stains cells transfected with human ULBP-2, human ULBP-5 or human RAET1L/ULBP-6 in flow cytometry. In direct ELISA, 11% cross‑reactivity with recombinant human ULBP5 is observed. It does not stain cells transfected with human ULBP-1 or human ULBP-3 in flow cytometry.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of ULBP-2/5/6 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human ULBP-2/5/6 PE‑conjugated Monoclonal Antibody (Catalog # FAB1298P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

ULBP-2/5/6

background_content

Background: ULBP-2/5/6 ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. The name ULBP derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The genes for ULBPs reside in a cluster of ten related genes, six of which encode potentially functional glycoproteins. ULBP-2 has also been described under the names RaeT1H (retinoic acid early transcript), NKG2DL2, and ALCAN-alpha. ULBP-5 also known as RaeT1G and ULBP-6 also known as RaeT1L. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-2, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance. Over aa 26-217, ULBP-2 shares 92% and 95% aa sequence identity with the human ULBP-5 and ULBP-6, respectively.

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Citations

1. Interleukin-15 stimulates natural killer cell-mediated killing of both human pancreatic cancer and stellate cells
   Authors: JRM Van Audena, J De Waele, E Marcq, J Van Loenho, E Lion, JMJ Van den Be, R Jesenofsky, A Masamune, G Roeyen, P Pauwels, F Lardon, M Peeters, ELJ Smits
   Oncotarget, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Activation of Innate and Adaptive Immunity by a Recombinant Human Cytomegalovirus Strain Expressing an NKG2D Ligand
   PLoS Pathog, 2016;12(12):e1006015.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Decitabine enhances anti-CD33 monoclonal antibody BI 836858-mediated natural killer ADCC against AML blasts
   Blood, 2016;127(23):2879-89.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Oncolytic reovirus enhances rituximab-mediated antibody-dependent cellular cytotoxicity against chronic lymphocytic leukaemia.
   Authors: Parrish C, Scott G, Migneco G, Scott K, Steele L, Ilett E, West E, Hall K, Selby P, Buchanan D, Varghese A, Cragg M, Coffey M, Hillmen P, Melcher A, Errington-Mais F
   Leukemia, 2015;29(9):1799-810.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Natural killer (NK) cells inhibit systemic metastasis of glioblastoma cells and have therapeutic effects against glioblastomas in the brain.
   Authors: Lee S, Kang W, Yoon Y, Jin J, Song H, Her J, Kang S, Hwang Y, Kang K, Joo K, Nam D
   BMC Cancer, 2015;15(1):1011.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. The NKG2D ligand ULBP2 is specifically regulated through an invariant chain-dependent endosomal pathway.
   Authors: Uhlenbrock F, Hagemann-Jensen M, Kehlet S, Andresen L, Pastorekova S, Skov S
   J Immunol, 2014;193(4):1654-65.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Natural killer cell-mediated shedding of ULBP2.
   Authors: Wang, Ruipeng, Sun, Peter D
   PLoS ONE, 2014;9(3):e91133.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Vesicular Stomatitis Virus Infection Promotes Immune Evasion by Preventing NKG2D-Ligand Surface Expression.
   Authors: Jensen H, Andresen L, Nielsen J, Christensen JP, Skov S
   PLoS ONE, 2011;6(8):e23023.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
   Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
   PLoS ONE, 2011;6(2):e16899.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Cytotoxicity of activated natural killer cells against pediatric solid tumors.
    Authors: Cho D, Shook DR, Shimasaki N
    Clin. Cancer Res., 2010;16(15):3901-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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