抗IgG 二次抗体(Anti-IgG, Goat, Rabbit-Poly, HRP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Goat
Label	HRP (Horseradish Peroxidase)
Immunogen	Goat IgG
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rat IgG, chicken IgY and human IgG is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	Use at a 1:1000 dilution.

Western Blot
	Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat Syntaxin1A Polyclonal Antibody (Catalog # AF7237)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF017).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

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Related Product & Information

Long Name	Immunoglobulin G
Background	IgG
background_content	Background: IgG R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

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Citations

1. Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes
   Authors: EC Bwalya, S Kim, J Fang, HMS Wijekoon, K Hosoya, M Okumura
   PLoS ONE, 2017;12(5):e0177144.
   Species: Canine
   Sample Type: Whole Cells
   Application: WB


2. Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma
   Authors: SC Sebag, OM Koval, JD Paschke, CJ Winters, OA Jaffer, R Dworski, FS Sutterwala, ME Anderson, IM Grumbach
   JCI Insight, 2017;2(3):e88297.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


3. Effect of 20-Hydroxyecdysone on Proteolytic Regulation in Skeletal Muscle Atrophy
   Authors: Apichart Suksamrarn
   In Vivo, 2016;30(6):869-877.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


4. Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail.
   Authors: Ahn J, Shin S, Suh Y, Park J, Hwang S, Lee K
   PLoS ONE, 2015;10(4):e0124768.
   Species: Avian - Quail
   Sample Type: Tissue Homogenates
   Application: WB


5. Identification and characterization of PERK activators by phenotypic screening and their effects on NRF2 activation.
   Authors: Xie W, Pariollaud M, Wixted W, Chitnis N, Fornwald J, Truong M, Pao C, Liu Y, Ames R, Callahan J, Solari R, Sanchez Y, Diehl A, Li H
   PLoS ONE, 2015;10(3):e0119738.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.
   Authors: Zhang X, Westerhoff M, Hart J
   Mod Pathol, 2015;28(5):654-61.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus.
   Authors: Zouk H, D'Hennezel E, Du X, Ounissi-Benkalha H, Piccirillo C, Polychronakos C
   Clin Exp Immunol, 2014;175(3):485-97.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Mechanical stretch inhibits lipopolysaccharide-induced keratinocyte-derived chemokine and tissue factor expression while increasing procoagulant activity in murine lung epithelial cells.
   Authors: Sebag S, Bastarache J, Ware L
   J Biol Chem, 2013;288(11):7875-84.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Lumican, an extracellular matrix proteoglycan, is a novel requisite for hepatic fibrosis.
   Authors: Krishnan A, Li X, Kao W, Viker K, Butters K, Masuoka H, Knudsen B, Gores G, Charlton M
   Lab Invest, 2012;92(12):1712-25.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


10. Possible role of Efnb1 protein, a ligand of Eph receptor tyrosine kinases, in modulating blood pressure.
    Authors: Wu Z, Luo H, Thorin E, Tremblay J, Peng J, Lavoie JL, Wang Y, Qi S, Wu T, Wu J
    J. Biol. Chem., 2012;287(19):15557-69.
    Species: N/A
    Sample Type: N/A
    Application: WB

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