抗CXCL1/GROα抗体(Anti-CXCL1/GROα, Mouse-Mono, PE antibody)

掲載日情報:2018/11/26 現在Webページ番号:32392

CXCL1/GROαに対する抗体(Anti-CXCL1/GROα, Mouse-Mono, PE )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2024年05月18日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-CXCL1/GROα, Mouse-Mono(20326), PE
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
説明文
別名:CXCL1,GRO alpha,KC,CINC-1
クローン:20326
Genbank No: 2919
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 Human 免疫動物 Mouse クラス IgG 標識 PE
交差性 Human 適用 FCM
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事

[在庫・価格 :2024年05月18日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-CXCL1/GROα, Mouse-Mono(20326), PE

文献数: 0

説明文 別名:CXCL1,GRO alpha,KC,CINC-1
クローン:20326
Genbank No: 2919
法規制等
保存条件 4℃,暗所保存,凍結禁止 法規備考
抗原種 Human 免疫動物 Mouse
交差性 Human 適用 FCM
標識 PE 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
関連記事



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Product Details

Species ReactivityHuman
LabelPhycoerythrin
ImmunogenE. coli-derived recombinant human CXCL1/GRO alpha /KC/CINC-1
SourceMonoclonal Mouse IgG2B Clone # 20326
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects human CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In Western blots, this antibody shows approximately 20% cross‑reactivity with recombinant human (rh) CXCL2/GRO beta and rhCXCL3/GRO gamma and no cross-reactivity with recombinant rat (rr) CINC‑1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhMIP-1 alpha, recombinant mouse (rm) MIP-1 alpha, rmMIP-1 beta, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP‑2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta.


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Applications and Data

 Recommended
Concentration
Sample
Intracellular Staining by Flow Cytometry10 µL/106 cellsSee below


Intracellular Staining by Flow Cytometry
Detection of CXCL1/GRO alpha /KC/CINC‑1 in Human Blood Monocytes by Flow Cytometry.
Activated human peripheral blood monocytes were stained with Mouse Anti-Human CXCL1/GRO alpha /KC/CINC‑1 PE‑conjugated Monoclonal Antibody (Catalog # IC275P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.


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Related Product & Information

Entrez Gene IDs2919 (Human); 14825 (Mouse); 81503 (Rat)
BackgroundCXCL1/GRO alpha/KC/CINC-1
background_contentBackground:
CXCL1/GRO alpha/KC/CINC-1
The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. Two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta have been identified, which share 90% and 86% amino acid sequence homology with GXCL1/GRO alpha, respectively. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. It remains to be seen if a unique GRO receptor(s) also exist. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity.


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