抗Human VEGF R2/KDR Fluorescein抗体(Anti-Human VEGF R2/KDR Fluorescein antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R2/KDR/Flk-1Ala20-Glu764Accession # P35968
Source	Monoclonal Mouse IgG1 Clone # 89106
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of VEGF R2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk‑1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB357F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Background

VEGF R2/KDR/Flk-1

background_content

Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGF R2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

目次に戻る

Citations

1. Leucocyte and Platelet-rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine
   Authors: R Di Liddo, T Bertalot, A Borean, I Pirola, A Argentoni, S Schrenk, C Cenzi, S Capelli, MT Conconi, PP Parnigotto
   J. Cell. Mol. Med., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Engineered ligand-based VEGFR antagonists with increased receptor binding affinity more effectively inhibit angiogenesis
   Authors: S Kapur, AP Silverman, AZ Ye, N Papo, D Jindal, MS Blumenkran, JR Cochran
   Bioeng Transl Med, 2017;2(1):81-91.
   Application: Flow


3. Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction
   PLoS ONE, 2016;11(8):e0160153.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments.
   Authors: De Biasi S, Cerri S, Bianchini E, Gibellini L, Persiani E, Montanari G, Luppi F, Carbonelli C, Zucchi L, Bocchino M, Zamparelli A, Vancheri C, Sgalla G, Richeldi L, Cossarizza A
   BMC Med, 2015;13(0):277.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
   Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
   J. Immunol., 2009;183(12):8176-85.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells.
   Authors: Xiong YQ, Sun HC, Zhang W
   Clin. Cancer Res., 2009;15(15):4838-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Heterochromatin Protein 1 Alpha (HP1alpha: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells.
   Authors: Maeng Y, Kwon J, Kim E, Kwon Y
   Stem Cells, 0;33(5):1512-22.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

目次に戻る