抗SOX10抗体(Anti-SOX10, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat SOX10aa 1-118
Source	Monoclonal Mouse IgG1 Clone # 20B7
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human and rat SOX10. Epitope-mapping experiments indicated that clone 20B7 recognizes a determinant in the first 65 amino acids, which contains sequences unique to SOX10 (1).

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Applications and Data

	Recommended Concentration	Sample
Immunohistochemistry	5-25 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry
	SOX10 in BG01V Human Embryonic Stem Cells. SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	SOX10 in Human Melanoma Tissue. SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Mouse Anti-Human/Rat SOX10 Monoclonal Antibody (Catalog # MAB2864) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Related Product & Information

Background	SOX10
background_content	Background: SOX10 SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

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Citations

1. Identification and functional analysis of SOX10 phosphorylation sites in melanoma
   Authors: JC Cronin, SK Loftus, LL Baxter, S Swatkoski, M Gucek, WJ Pavan
   PLoS ONE, 2018;13(1):e0190834.
   Species: Human
   Sample Type: Cell Lysates
   Application: IP


2. Analysis of induced pluripotent stem cells carrying 22q11.2 deletion
   Authors: T Yoshikawa
   Transl Psychiatry, 2016;6(11):e934.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair.
   Authors: Xu W, Sun Y, Zhang J, Xu K, Pan L, He L, Song Y, Njunge L, Xu Z, Chiang M, Sung K, Chuong C, Yang L
   Stem Cells Dev, 2015;24(7):857-68.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC Fluorescence


4. Oligodendroglial maturation is dependent on intracellular protein shuttling.
   Authors: Gottle P, Sabo J, Heinen A, Venables G, Torres K, Tzekova N, Parras C, Kremer D, Hartung H, Cate H, Kury P
   J Neurosci, 2015;35(3):906-19.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


5. Modulation of lipopolysaccharide-induced neuronal response by activation of the enteric nervous system.
   Authors: Coquenlorge S, Duchalais E, Chevalier J, Cossais F, Rolli-Derkinderen M, Neunlist M
   J Neuroinflammation, 2014;11(1):202.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


6. HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia.
   Authors: Jacob C, Lotscher P, Engler S, Baggiolini A, Varum Tavares S, Brugger V, John N, Buchmann-Moller S, Snider P, Conway S, Yamaguchi T, Matthias P, Sommer L, Mantei N, Suter U
   J Neurosci, 2014;34(17):6112-22.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


7. HDAC1 and HDAC2 control the transcriptional program of myelination and the survival of Schwann cells.
   Authors: Jacob C, Christen CN, Pereira JA, Somandin C, Baggiolini A, Lotscher P, Ozcelik M, Tricaud N, Meijer D, Yamaguchi T, Matthias P, Suter U
   Nat. Neurosci., 2011;14(4):429-36.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


8. A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin.
   Authors: Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S
   Stem Cells, 2007;26(1):163-72.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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