抗Nestin抗体(Anti-Nestin, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant rat NestinMet544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)Accession # EDM00749
Source	Monoclonal Mouse IgG2A Clone # 307501
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse and rat Nestin in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of Nestin in Mouse Cortical Stem Cells by Flow Cytometry. Mouse cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
	Detection of Nestin in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE‑conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

Nestin

background_content

Background: Nestin Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14). Over amino acids (aa) 544-820, rat Nestin shares 75% and 38% sequence identity with mouse and human Nestin, respectively.

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Citations

1. The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals
   Authors: Y Wang, X Ying, H Xu, H Yan, X Li, H Tang
   Int J Nanomedicine, 2017;12(0):1369-1384.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
   Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
   Cell Death Discov, 2017;3(0):16105.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile
   J Immunol, 2016;196(11):4760-70.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
   Authors: Hu S, Rotschafer J, Lokensgard J, Cheeran M
   PLoS ONE, 2014;9(8):e105219.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
   Authors: Mutnal MB, Cheeran MC, Hu S
   PLoS ONE, 2011;6(1):e16211.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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