抗KLF4抗体(Anti-KLF4, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse KLF4Ala11-Phe483Accession # Q60793
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse KLF4 in direct ELISAs and Western blots. In these formats, approximately 10% cross-reactivity with recombinant human (rh) KLF4 is observed, and less than 2% cross-reactivity with rhKLF2 and rhKLF5 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse KLF4
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Chromatin Immunoprecipitation (ChIP)
	Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The B2R promoter was detected by standard PCR.
Immunocytochemistry
	KLF4 in D3 Mouse Cell Line. KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Long Name	Kruppel-Like Factor 4
Background	KLF4
background_content	Background: KLF4 KLF4 belongs to the KLF family of transcription factors that regulate several cell functions. It is highly expressed in differentiated epithelial cells of the gastrointestinal tract. Human and mouse KLF4 share 91% amino acid sequence homology.

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Citations

1. Kr�ppel-like factor 4 is a negative regulator of STAT3-induced glomerular epithelial cell proliferation
   Authors: CC Estrada, P Paladugu, Y Guo, J Pace, MP Revelo, DJ Salant, SJ Shankland, VD D'Agati, A Mehrotra, S Cardona, AB Bialkowska, VW Yang, JC He, SK Mallipattu
   JCI Insight, 2018;3(12):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. RNF12 X-Linked Intellectual Disability Mutations Disrupt E3 Ligase Activity and Neural Differentiation
   Authors: F Bustos, A Segarra-Fa, VK Chaugule, L Brandenbur, E Branigan, R Toth, T Macartney, A Knebel, RT Hay, H Walden, GM Findlay
   Cell Rep, 2018;23(6):1599-1611.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Cell Autonomous and Non-cell Autonomous Regulation of SMC Progenitors in Pulmonary Hypertension
   Authors: AQ Sheikh, FZ Saddouk, A Ntokou, R Mazurek, DM Greif
   Cell Rep, 2018;23(4):1152-1165.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function
   Authors: C Antognelli, E Trapani, S Delle Mona, A Perrelli, C Fornelli, F Retta, P Cassoni, VN Talesa, SF Retta
   Data Brief, 2018;16(0):929-938.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
   Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
   Cell Death Discov, 2017;3(0):16105.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
   Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
   J. Clin. Invest., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Integrated analysis of hematopoietic differentiation outcomes and molecular characterization reveals unbiased differentiation capacity and minor transcriptional memory in HPC/HSC-iPSCs
   Authors: S Gao, X Hou, Y Jiang, Z Xu, T Cai, J Chen, G Chang
   Stem Cell Res Ther, 2017;8(1):13.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


8. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
   Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
   Oxid Med Cell Longev, 2017;2017(0):4745252.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


9. WNT10A mutation causes ectodermal dysplasia by impairing progenitor cell proliferation and KLF4-mediated differentiation
   Authors: M Xu, J Horrell, M Snitow, J Cui, H Gochnauer, CM Syrett, S Kallish, JT Seykora, F Liu, D Gaillard, JP Katz, KH Kaestner, B Levin, C Mansfield, JE Douglas, BJ Cowart, M Tordoff, F Liu, X Zhu, LA Barlow, AI Rubin, JA McGrath, EE Morrisey, EY Chu, SE Millar
   Nat Commun, 2017;8(0):15397.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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