抗Integrin αVβ3抗体(Anti-Integrin αVβ3, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Human osteoclasts
Source	Monoclonal Mouse IgG1 Clone # 23C6
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Integrin alpha V beta 3.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	HUVEC human umbilical vein endothelial cells
Immunohistochemistry	Davies, J. et al. (1989) J. Cell Biol. 109:1817.
Immunoprecipitation	Davies, J. et al. (1989) J. Cell Biol. 109:1817.
Adhesion Blockade	Horton, M.A. et al. (1991) Exp. Cell Res. 195:368. Bates, R.C. et al. (1998) Cell Adhes. Commun. 6:21.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

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Related Product & Information

Entrez Gene IDs	3685 (Human)
Background	Integrin alpha V beta 3
background_content	Background: Integrin alpha V beta 3 Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.

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Citations

1. Osteopontin-integrin engagement induces HIF-1?-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer
   Authors: CS Fan, WS Chen, LL Chen, CC Chen, YT Hsu, KV Chua, HD Wang, TS Huang
   Oncotarget, 2018;9(4):4998-5015.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


2. HLA Class II-Triggered Signaling Cascades Cause Endothelial Cell Proliferation and Migration: Relevance to Antibody-Mediated Transplant Rejection
   Authors: YP Jin, NM Valenzuela, X Zhang, E Rozengurt, EF Reed
   J. Immunol., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


3. Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo
   Authors: HA Mena, PR Zubiry, B Dizier, M Schattner, C Boisson-Vi, S Negrotto
   Stem Cell Res Ther, 2018;9(1):120.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. AlphaVBeta3 Integrin expression within uterine endometrium in unexplained infertility: a prospective cohort study
   Authors: A Elnaggar, AH Farag, ME Gaber, MA Hafeez, MS Ali, AM Atef
   BMC Womens Health, 2017;17(1):90.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin-embedded


5. cRGD peptide installation on cisplatin-loaded nanomedicines enhances efficacy against locally advanced head and neck squamous cell carcinoma bearing cancer stem-like cells
   Authors: K Miyano, H Cabral, Y Miura, Y Matsumoto, Y Mochida, H Kinoh, K Iwata, O Nagano, H Saya, N Nishiyama, K Kataoka, T Yamasoba
   J Control Release, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy.
   Authors: Su C, Chiang Y, Huang C, Hsu C, Fong Y, Tang C
   J Immunol, 2015;195(7):3355-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
   Authors: Kasonga A, Deepak V, Kruger M, Coetzee M
   PLoS ONE, 2015;10(4):e0125145.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Not Specified


8. C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
   Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
   Sci Rep, 2015;5(0):9275.
   


9. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
   Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
   Cell Commun Signal, 2014;12(0):36.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis.
    Authors: DeNiro M, Al-Mohanna F, Alsmadi O, Al-Mohanna F
    PLoS ONE, 2013;8(3):e59021.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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