抗Human IL-22 Phycoerythrin抗体(Anti-Human IL-22 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant human IL-22Ala34-Ile179Accession # Q9GZX6
Source	Monoclonal Mouse IgG1 Clone # 142928
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑22 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Ca++ Ionomycin, LPS, and Recombinant Human IL‑23 (Catalog # 1290-IL) were stained with Mouse Anti-Human IL‑22 PE‑conjugated Monoclonal Antibody (Catalog # IC7821P) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-22

background_content

Background: IL-22 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

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Citations

1. A20 upregulation during treated HIV disease is associated with intestinal epithelial cell recovery and function
   Authors: AS Chitre, MG Kattah, YY Rosli, M Pao, M Deswal, SG Deeks, PW Hunt, M Abdel-Mohs, LJ Montaner, CC Kim, A Ma, M Somsouk, JM McCune
   PLoS Pathog., 2018;14(3):e1006806.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Skin-infiltrating, interleukin-22-producing T cells differentiate pediatric psoriasis from adult psoriasis
   Authors: KM Cordoro, M Hitraya-Lo, K Taravati, PM Sandoval, E Kim, J Sugarman, ML Pauli, W Liao, MD Rosenblum
   J. Am. Acad. Dermatol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Enhancing adoptive cancer immunotherapy with V?2V?2 T cells through pulse zoledronate stimulation
   Authors: MH Nada, H Wang, G Workalemah, Y Tanaka, CT Morita
   J Immunother Cancer, 2017;5(0):9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. High frequency of intestinal TH17 cells correlates with microbiota alterations and disease activity in multiple sclerosis
   Authors: I Cosorich, G Dalla-Cost, C Sorini, R Ferrarese, MJ Messina, J Dolpady, E Radice, A Mariani, PA Testoni, F Canducci, G Comi, V Martinelli, M Falcone
   Sci Adv, 2017;3(7):e1700492.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human non-fibrotic and fibrotic livers
   Authors: M Forkel, L Berglin, E Kekäläinen, A Carlsson, E Svedin, J Michaëlsso, M Nagasawa, JS Erjefält, M Mori, M Flodström-, A Bergquist, HG Ljunggren, M Westgren, U Lindforss, D Friberg, C Jorns, E Ellis, NK Björkström, J Mjösberg
   Eur. J. Immunol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. IL-22-producing CD4+T cells in the treatment response of rheumatoid arthritis to combination therapy with methotrexate and leflunomide
   Authors: W Zhong, L Zhao, T Liu, Z Jiang
   Sci Rep, 2017;7(0):41143.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Expansion of Th17 Cells by Human Mast Cells Is Driven by Inflammasome-Independent IL-1?
   Authors: J Suurmond, KL Habets, AL Dorjée, TW Huizinga, RE Toes
   J. Immunol, 2016;197(11):4473-4481.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
   Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
   J Exp Med, 2015;212(5):619-31.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
   Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
   Eur J Immunol, 2015;45(2):442-51.
   Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
   Sample Type: Whole Cells
   Application: Flow


10. A higher frequency of circulating IL-22(+)CD4(+) T cells in Chinese patients with newly diagnosed Hashimoto's thyroiditis.
    Authors: Guo H, Peng D, Yang X, Wang Y, Xu B, Ni J, Meng W, Jiang Y
    PLoS ONE, 2014;9(1):e84545.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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