抗Human IL-22 Allophycocyanin抗体(Anti-Human IL-22 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Allophycocyanin
Immunogen	E. coli-derived recombinant human IL-22Ala34-Ile179Accession # Q9GZX6
Source	Monoclonal Mouse IgG1 Clone # 142928
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑22 in Human PBMCs stimulated to induce Th17 Cells by Flow Cytometry. CD4+ Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th17 cells with 10 ug/mL Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100), 5 ug/mL Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342), 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B), 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL), 20 ng/mL Recombinant Human IL‑23 (Catalog # 1290-IL), 40 ng/mL Recombinant Human IL‑6 (Catalog # 206-IL), and 10 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) for 5 to 7 days, then re-stimulated with 50 ng/mL PMA and 200 ng/mL Calcium ionomycin for 2 to 4 hours or (B) resting CD4+ PBMCs were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human IL‑22 APC‑conjugated Monoclonal Antibody (Catalog # IC7821A). Quadrant markers were set based on isotype control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

目次に戻る

Related Product & Information

Background

IL-22

background_content

Background: IL-22 Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

目次に戻る

Citations

1. Mycobacterium tuberculosis-Specific IL-21+IFN-gamma+CD4+ T Cells Are Regulated by IL-12.
   Authors: Li L, Jiang Y, Lao S, Yang B, Yu S, Zhang Y, Wu C
   PLoS ONE, 2016;11(1):e0147356.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Trained immunity in newborn infants of HBV-infected mothers.
   Authors: Hong M, Sandalova E, Low D, Gehring A, Fieni S, Amadei B, Urbani S, Chong Y, Guccione E, Bertoletti A
   Nat Commun, 2015;6(0):6588.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Initiation of ART during early acute HIV infection preserves mucosal Th17 function and reverses HIV-related immune activation.
   Authors: Schuetz A, Deleage C, Sereti I, Rerknimitr R, Phanuphak N, Phuang-Ngern Y, Estes J, Sandler N, Sukhumvittaya S, Marovich M, Jongrakthaitae S, Akapirat S, Fletscher J, Kroon E, Dewar R, Trichavaroj R, Chomchey N, Douek D, O Connell R, Ngauy V, Robb M, Phanuphak P, Michael N, Excler J, Kim J, de Souza M, Ananworanich J
   PLoS Pathog, 2014;10(12):e1004543.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. CX(3)CL1 (fractalkine) and its receptor CX(3)CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin.
   Authors: Staumont-Salle D, Fleury S, Lazzari A, Molendi-Coste O, Hornez N, Lavogiez C, Kanda A, Wartelle J, Fries A, Pennino D, Mionnet C, Prawitt J, Bouchaert E, Delaporte E, Glaichenhaus N, Staels B, Julia V, Dombrowicz D
   J Exp Med, 2014;211(6):1185-96.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
   Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
   J Allergy Clin Immunol, 2013;131(2):562-70.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
   Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
   J Allergy Clin Immunol, 2013;131(4):1117-29, 1129.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
   Authors: Tang, Xin-Zi, Jo, Juandy, Tan, Anthony, Sandalova, Elena, Chia, Adeline, Tan, Kai Chah, Lee, Kang Hoe, Gehring, Adam J, De Libero, Gennaro, Bertoletti, Antonio
   J Immunol, 2013;190(7):3142-52.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope.
   Authors: Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R
   PLoS Pathog, 2012;8(11):e1003042.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
   Authors: Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C
   Immunology, 2011;132(4):540-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection.
    Authors: Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY
    Scand. J. Immunol., 2011;73(4):330-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

目次に戻る