抗Human/Mouse SSEA-4 Allophycocyanin抗体(Anti-Human/Mouse SSEA-4 Allophycocyanin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Mouse
Label	Allophycocyanin
Immunogen	2120Ep human embryonal carcinoma cell line
Source	Monoclonal Mouse IgG3 Clone # MC-813-70
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Recognizes a carbohydrate epitope of SSEA-4 (1, 2).

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 APC‑conjugated Monoclonal Antibody (Catalog # FAB1435A, filled histogram) or isotype control antibody (Catalog # IC007A, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Background

SSEA-4

background_content

Background: SSEA-4 SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

目次に戻る

Citations

1. Establishment of an induced pluripotent stem (iPS) cell line from dermal fibroblasts of an asymptomatic patient with dominant PRPF31 mutation
   Authors: A Terray, V Fort, A Slembrouck, C Nanteau, JA Sahel, S Reichman, I Audo, O Goureau
   Stem Cell Res, 2017;25(0):26-29.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimer's disease (ASUi001-A) and non-demented control (ASUi002-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant
   Authors: N Brookhouse, P Zhang, R Caselli, JJ Kim, DA Brafman
   Stem Cell Res, 2017;24(0):160-163.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Generation of an induced pluripotent stem cell (iPSC) line from a patient with autosomal dominant retinitis pigmentosa due to a mutation in the NR2E3 gene
   Authors: A Terray, A Slembrouck, C Nanteau, C Chondroyer, C Zeitz, JA Sahel, I Audo, S Reichman, O Goureau
   Stem Cell Res, 2017;24(0):1-4.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Generation of hiPSTZ16 (ISMMSi003-A) cell line from normal human foreskin fibroblasts
   Authors: M Dejosez, TP Zwaka
   Stem Cell Res, 2017;26(0):44-46.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Prospectively Isolated Human Bone Marrow Cell-Derived MSCs Support Primitive Human CD34-Negative Hematopoietic Stem Cells.
   Authors: Matsuoka Y, Nakatsuka R, Sumide K, Kawamura H, Takahashi M, Fujioka T, Uemura Y, Asano H, Sasaki Y, Inoue M, Ogawa H, Takahashi T, Hino M, Sonoda Y
   Stem Cells, 2015;33(5):1554-65.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells.
   Authors: Zhu H, Hu S, Baker J
   Stem Cells, 2014;32(8):2098-110.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies.
   Authors: Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S
   PLoS ONE, 2013;8(1):e55319.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Evaluation of ABCG2 expression in human embryonic stem cells: crossing the same river twice?
   Authors: Sarkadi B, Orban TI, Szakacs G, Varady G, Schamberger A, Erdei Z, Szebenyi K, Homolya L, Apati A
   Stem Cells, 2010;28(1):174-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. High concentration of synthetic serum, stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem cells.
   Authors: Lee JY, Lee JE, Kim DK, Yoon TK, Chung HM, Lee DR
   Fertil. Steril., 2010;93(3):976-85.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Expression and functional analysis of G1 to S regulatory components reveals an important role for CDK2 in cell cycle regulation in human embryonic stem cells.
    Authors: Neganova I, Zhang X, Atkinson S, Lako M
    Oncogene, 2009;28(1):20-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

目次に戻る