抗SOX9抗体(Anti-SOX9, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human SOX9Met1-Lys151Accession # P48436
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) SOX10 is observed, and less than 15% rhSOX11, rhSOX12, and rhSOX15 is observed, and less than 5% cross-reactivity with rhSOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX8, rhSOX13, rhSOX17, rhSOX18, rhSOX21, and rhSOX30 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human SOX9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	SOX9 in HEK293 Human Cell Line. SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	SOX9 in BG01V Human Embryonic Stem Cells. SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western
	Detection of Human SOX9 by Simple WesternTM. Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Transcription Factor SOX9
Background	SOX9
background_content	Background: SOX9 SOX9 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX9 is expressed in mesenchymal progenitors that give rise to chondrocytes and osteoblasts. It is also expressed in the central nervous system, neural crest, intestine, pancreas, and testis. Mutations in SOX9 are associated with defects in sex determination, cartilage and bone development, as well as abnormalities of the heart, kidneys, brain, gut, and pancreas.

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Citations

1. Restorative effects of human neural stem cell grafts on the primate spinal cord
   Authors: ES Rosenzweig, JH Brock, P Lu, H Kumamaru, EA Salegio, K Kadoya, JL Weber, JJ Liang, R Moseanko, S Hawbecker, JR Huie, LA Havton, YS Nout-Lomas, AR Ferguson, MS Beattie, JC Bresnahan, MH Tuszynski
   Nat. Med., 2018;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors
   Authors: II Aigha, B Memon, AK Elsayed, EM Abdelalim
   Stem Cell Res Ther, 2018;9(1):83.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells
   Authors: M Zhang, J Ngo, F Pirozzi, YP Sun, A Wynshaw-Bo
   Stem Cell Res Ther, 2018;9(1):67.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Meis1 coordinates cerebellar granule cell development by regulating Pax6 transcription, BMP signaling and Atoh1 degradation
   Authors: T Owa, S Taya, S Miyashita, M Yamashita, T Adachi, K Yamada, M Yokoyama, S Aida, T Nishioka, YU Inoue, R Goitsuka, T Nakamura, T Inoue, K Kaibuchi, M Hoshino
   J. Neurosci., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


5. Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
   Authors: B Memon, M Karam, S Al-Khawaga, EM Abdelalim
   Stem Cell Res Ther, 2018;9(1):15.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. SOX9 is an astrocyte-specific nuclear marker in the adult brain outside the neurogenic regions
   Authors: W Sun, A Cornwell, J Li, S Peng, MJ Osorio, NA Su Wanga, A Benraiss, N Lou, SA Goldman, M Nedergaard
   J. Neurosci, 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


7. Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preB�tzinger complex
   Authors: A Kottick, CA Martin, CA Del Negro
   Physiol Rep, 2017;5(11):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos
   Authors: WJ Lee, S Chatterjee, SP Yap, SL Lim, X Xing, P Kraus, W Sun, X Hu, V Sivakamasu, HY Chan, PR Kolatkar, S Prabhakar, T Lufkin
   Biomed Res Int, 2017;2017(0):8932583.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ChIP


9. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis
   Authors: C Lin, E Yao, K Zhang, X Jiang, S Croll, K Thompson-P, PT Chuang
   Elife, 2017;6(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate
    Authors: Emma L Rawlins
    Development, 2016;143(20):3686-3699.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

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