抗Nanog抗体(Anti-Nanog, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse NanogTrp154-Leu262Accession # Q80Z64
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects recombinant mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	Yang, W. et al. (2014) Nat. Comm. 5:3818
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of Nanog in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Immunocytochemistry
	Nanog in D3 Mouse Cell Line. Nanog was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Related Product & Information

Background	Nanog
background_content	Background: Nanog Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency(1-3).

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Citations

1. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
   Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
   Cell Stem Cell, 2018;22(4):543-558.e12.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
   Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
   Cell Death Discov, 2017;3(0):16105.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Lysine-specific histone demethylase 1 inhibition promotes reprogramming by facilitating the expression of exogenous transcriptional factors and metabolic switch
   Sci Rep, 2016;6(0):30903.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Fresh


5. ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation
   Stem Cell Res, 2016;17(2):222-227.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Not Specified


6. FBXL10 protects Polycomb-bound genes from hypermethylation.
   Authors: Boulard M, Edwards J, Bestor T
   Nat Genet, 2015;47(5):479-85.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Not Specified


7. Loss of tenomodulin results in reduced self-renewal and augmented senescence of tendon stem/progenitor cells.
   Authors: Alberton P, Dex S, Popov C, Shukunami C, Schieker M, Docheva D
   Stem Cells Dev, 2015;24(5):597-609.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IF


8. Embryonic Stem Cell Growth Factors Regulate eIF2alpha Phosphorylation.
   Authors: Friend K, Brooks H, Propson N, Thomson J, Kimble J
   PLoS ONE, 2015;10(9):e0139076.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Cyclin-dependent kinase-mediated Sox2 phosphorylation enhances the ability of Sox2 to establish the pluripotent state.
   Authors: Ouyang J, Yu W, Liu J, Zhang N, Florens L, Chen J, Liu H, Washburn M, Pei D, Xie T
   J Biol Chem, 2015;290(37):22782-94.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC Fluorescence


10. Acute loss of Cited2 impairs Nanog expression and decreases self-renewal of mouse embryonic stem cells.
    Authors: Kranc K, Oliveira D, Armesilla-Diaz A, Pacheco-Leyva I, Catarina Matias A, Luisa Escapa A, Subramani C, Wheadon H, Trindade M, Nichols J, Kaji K, Enver T, Braganca J
    Stem Cells, 2015;33(3):699-712.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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