抗Nestin抗体(Anti-Nestin, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat NestinMet544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)Accession # EDM00749
Source	Monoclonal Mouse IgG2A Clone # 307501
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse and rat Nestin in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Rat Nestin
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

Nestin in Rat Cortical Stem Cells. Nestin was detected in immersion fixed undifferentiated rat cortical stem cells using Mouse Anti‑Rat Nestin Monoclonal Antibody (Catalog # MAB2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Nestin

background_content

Background: Nestin Nestin is a class VI intermediate filament protein (1,2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells.(4) Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway.(2) Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

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Citations

1. Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy
   Authors: SB Ross, ST Fraser, RD Bagnall, C Semsarian
   Stem Cell Res, 2017;20(0):76-79.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
   Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
   Oncotarget, 2017;8(17):28074-28092.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. MUNC18-1 gene abnormalities are involved in neurodevelopmental disorders through defective cortical architecture during brain development
   Authors: N Hamada, I Iwamoto, H Tabata, KI Nagata
   Acta Neuropathol Commun, 2017;5(1):92.
   Species: Primate
   Sample Type: Whole Cells
   Application: ICC


4. The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking neural stem cell-derived extracellular vesicles
   Authors: A Yoshimura, N Adachi, H Matsuno, M Kawamata, Y Yoshioka, H Kikuchi, H Odaka, T Numakawa, H Kunugi, T Ochiya, Y Tamai
   Dis Model Mech, 2017;0(0):.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


5. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
   Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
   Cell Death Discov, 2017;3(0):16105.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. An Immortalized Human Dorsal Root Ganglia Cell Line Provides a Novel Context to Study Herpes Simplex Virus Type-1 Latency and Reactivation
   Authors: NM Thellman, C Botting, Z Madaj, SJ Triezenber
   J. Virol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. The PD-1/B7-H1 pathway modulates the natural killer cells versus mouse glioma stem cells.
   Authors: Huang B, Zhan Y, Zong W, Yu C, Li J, Qu Y, Han S
   PLoS ONE, 2015;10(8):e0134715.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


8. The protein phosphatase activity of PTEN is essential for regulating neural stem cell differentiation.
   Authors: Lyu J, Yu X, He L, Cheng T, Zhou J, Cheng C, Chen Z, Cheng G, Qiu Z, Zhou W
   Mol Brain, 2015;8(0):26.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC - PFA fixed


9. Nestin delineates pancreatic cancer stem cells in metastatic foci of NOD/Shi-scid IL2Rgamma(null) (NOG) mice.
   Authors: Matsuda Y, Yoshimura H, Ueda J, Naito Z, Korc M, Ishiwata T
   Am J Pathol, 2014;184(3):674-85.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


10. Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm.
    Authors: Engels M, Rajarajan K, Feistritzer R, Sharma A, Nielsen U, Schalij M, de Vries A, Pijnappels D, Wu S
    Stem Cells, 2014;32(6):1493-502.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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