抗Mouse Siglec-F抗体(Anti-Mouse Siglec-F antibody)
掲載日情報:2018/11/26 現在Webページ番号:31660
Mouse Siglec-Fに対する抗体(Anti-Mouse Siglec-F )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 :2025年04月26日 10時15分現在]
詳細 | 商品名 |
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文献数 | ||
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Anti-Mouse Siglec-F MAb (Clone 238047) |
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1 | |||
Anti-Mouse Siglec-F MAb (Clone 238047) |
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1 | |||
Anti-Mouse Siglec-F MAb (Clone 238047) |
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1 | |||
[在庫・価格 :2025年04月26日 10時15分現在]
Anti-Mouse Siglec-F MAb (Clone 238047)
文献数: 1
- 商品コード:MAB17061-100
- メーカー:RSD
- 包装:100μg
- 価格:¥101,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
Anti-Mouse Siglec-F MAb (Clone 238047)
文献数: 1
- 商品コード:MAB17061-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:1個
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
Anti-Mouse Siglec-F MAb (Clone 238047)
文献数: 1
- 商品コード:MAB17061-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
追加しました。
Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Siglec-FAsp18-Thr437Accession # Q920G3 |
Source | Monoclonal Rat IgG2A Clone # 238047 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse Siglec-F in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Siglec-2 or -3, or recombinant human Siglec-5, -7, -9, -10, or -11 is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Neutralization | Measured by its ability to neutralize Siglec‑F-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 5 µg/mL Recombinant Mouse Siglec‑F Fc Chimera. |
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Related Product & Information
Long Name | Sialic Acid Binding Ig-like Lectin F |
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Entrez Gene IDs | 233186 (Mouse) |
Background | Siglec-F |
background_content | Background: Siglec-F Siglecs (1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins (2) belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding (3), followed by varying numbers of Ig-like C2-type domains (1, 4). Eleven human Siglecs have been cloned and characterized (1, 4). They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec 5 to 11 (4‑6). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (4, 5). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system. Mouse Siglec-F cDNA encodes a 569 amino acid polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, three Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (7). The expression of Siglec-F is restricted to the cells of myelomonocytic lineage. Mouse Siglec-F is likely an ortholog of human Siglec-5. Unlike many human CD33-related Siglecs, which show similar binding to both alpha 2,3- and alpha 2,6-linked sialic acids, mouse Siglec-F preferentially recognize alpha 2,3-linked sialic acid. |
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Citations
- Role of Eosinophils and Tumor Necrosis Factor Alpha in Interleukin-25-Mediated Protection from Amebic Colitis
Authors: Z Noor, K Watanabe, MM Abhyankar, SL Burgess, EL Buonomo, CA Cowardin, WA Petri
MBio, 2017;8(1):.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F
PLoS Pathog, 2015;11(2):e1004607.
Species: Mouse
Sample Type: In Vivo
Application: Bioassay - Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis.
Authors: Chu V, Beller A, Rausch S, Strandmark J, Zanker M, Arbach O, Kruglov A, Berek C
Immunity, 2014;40(4):582-93.
Species: Mouse
Sample Type: In Vivo
Application: In vivo
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