抗MIP-1α抗体(Anti-MIP-1α, Rat-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92Accession # P10855
Source	Monoclonal Rat IgG2A Clone # 39624
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed. Neutralizes the biological activity of recombinant mouse CCL3 and will not block the biological activity of recombinant human CCL3.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of CCL3/MIP‑1 alpha in LPS-stimulated RAW 264.7 Mouse Cell Line by Flow Cytometry. LPS-stimulated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCL3/MIP‑1 alpha PE‑conjugated Monoclonal Antibody (Catalog # IC450P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

CCL3/MIP-1 alpha

background_content

Background: CCL3/MIP-1 alpha The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines. Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

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Citations

1. Long-Term Persistence of Exhausted CD8�T Cells in Chronic Infection Is Regulated by MicroRNA-155
   Authors: E Stelekati, Z Chen, S Manne, M Kurachi, MA Ali, K Lewy, Z Cai, K Nzingha, LM McLane, JL Hope, AJ Fike, PD Katsikis, EJ Wherry
   Cell Rep, 2018;23(7):2142-2156.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. miR-150 Regulates Memory CD8�T Cell Differentiation via c-Myb
   Authors: Z Chen, E Stelekati, M Kurachi, S Yu, Z Cai, S Manne, O Khan, X Yang, EJ Wherry
   Cell Rep, 2017;20(11):2584-2597.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. Prime-Boost Immunization Eliminates Metastatic Colorectal Cancer by Producing High-Avidity Effector CD8(+) T Cells
   Authors: B Xiang, TR Baybutt, L Berman-Boo, MS Magee, SA Waldman, VY Alexeev, AE Snook
   J. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. GUCY2C-directed CAR-T cells oppose colorectal cancer metastases without autoimmunity
   Authors: Adam E Snook
   Oncoimmunology, 2016;5(10):e1227897.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow

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