抗Human/Mouse SSEA-1 Phycoerythrin抗体(Anti-Human/Mouse SSEA-1 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Phycoerythrin
Immunogen	F9 mouse teratocarcinoma stem cells
Source	Monoclonal Mouse IgM Clone # MC-480
Purification	IgM-specific Affinity-purified from hybridoma culture supernatant
Specificity	Detects human and mouse SSEA-1.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

SSEA-1

background_content

Background: SSEA-1 Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

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Citations

1. A dual molecular analogue tuner for dissecting protein function in mammalian cells
   Nat Commun, 2016;7(0):11742.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
   Authors: Sugawa F, Arauzo-Bravo M, Yoon J, Kim K, Aramaki S, Wu G, Stehling M, Psathaki O, Hubner K, Scholer H
   EMBO J, 2015;34(8):1009-24.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. 5-Aminoimidazole-4-carboxyamide ribonucleoside induces G(1)/S arrest and Nanog downregulation via p53 and enhances erythroid differentiation.
   Authors: Chae HD, Lee MR, Broxmeyer HE
   Stem Cells, 2012;30(2):140-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
   Authors: Klim JR, Li L, Wrighton PJ
   Nat. Methods, 2010;7(12):989-94.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro.
   Authors: Hayashi K, Surani MA
   Development, 2009;136(21):3549-56.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
   Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
   Biotechnol. Bioeng., 2009;102(6):1636-44.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
   Authors: Fong H, Hohenstein KA, Donovan PJ
   Stem Cells, 2008;26(8):1931-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency.
   Authors: Hanna J, Markoulaki S, Schorderet P, Carey BW, Beard C, Wernig M, Creyghton MP, Steine EJ, Cassady JP, Foreman R, Lengner CJ, Dausman JA, Jaenisch R
   Cell, 2008;133(2):250-64.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells.
   Authors: Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS
   Stem Cells, 2007;25(6):1393-401.
   Species: Rat
   Sample Type: Whole Cells
   Application: Flow

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