抗CREB抗体(Anti-CREB , Phosphorylated, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Phosphopeptide containing human, mouse, and rat CREB S133 site
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat CREB when phosphorylated at S133 in Western blots.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	10 µg/mL	See below

Western Blot
	Detection of Human Phospho-CREB (S133) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CREB (S133) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2510), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CREB (S133) at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human Phospho-CREB (S133) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-CREB (S133) at approximately 54 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CREB (S133) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2510). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

目次に戻る

Related Product & Information

Long Name	cAMP Response Element-binding Protein
Background	CREB
background_content	Background: CREB The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

目次に戻る

Citations

1. A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
   Authors: Dumitru CA, Fechner MK, Hoffmann TK
   J. Leukoc. Biol., 2012;91(4):591-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Mal mediates TLR-induced activation of CREB and expression of IL-10.
   Authors: Mellett M, Atzei P, Jackson R, O'Neill LA, Moynagh PN
   J. Immunol., 2011;186(8):4925-35.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: ChIP


3. The kinase p38 alpha serves cell type-specific inflammatory functions in skin injury and coordinates pro- and anti-inflammatory gene expression.
   Authors: Kim C, Sano Y, Todorova K, Carlson BA, Arpa L, Celada A, Lawrence T, Otsu K, Brissette JL, Arthur JS, Park JM
   Nat. Immunol., 2008;9(9):1019-27.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: ChIP


4. Antiinflammatory cAMP signaling and cell migration genes co-opted by the anthrax bacillus.
   Authors: Kim C, Wilcox-Adelman S, Sano Y, Tang WJ, Collier RJ, Park JM
   Proc. Natl. Acad. Sci. U.S.A., 2008;105(16):6150-5.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Soluble EP2 neutralizes prostaglandin E2-induced cell signaling and inhibits osteolytic tumor growth.
   Authors: Takahashi T, Uehara H, Bando Y, Izumi K
   Mol. Cancer Ther., 2008;7(9):2807-16.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
   Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
   Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7281-6.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Proteinase-activated receptor-2 induces cyclooxygenase-2 expression through beta-catenin and cyclic AMP-response element-binding protein.
   Authors: Wang H, Wen S, Bunnett NW, Leduc R, Hollenberg MD, MacNaughton WK
   J. Biol. Chem., 2007;283(2):809-15.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

目次に戻る