抗GFAP抗体(Anti-GFAP, Sheep-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GFAPLeu292-Met432Accession # P14136
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and rat GFAP in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	0.1-2 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human and Rat GFAP by Western Blot. Western blot shows lysates of rat cortical stem cells, rat brain tissue, human brain (cortex) tissue, and human brain (hypothalamus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFAP at approximately 35-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	GFAP in Rat Astrocytes. GFAP was detected in immersion fixed rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	beta ‑III Tubulin in Rat Cortical Neurons and GFAP in Rat Astrocytes. beta ‑III Tubulin was detected in rat cortical neurons using 5 µg/mL Mouse Anti-neuron-specific Mouse beta ‑III Tubulin Monoclonal (clone TuJ‑1) Antibody (Catalog # MAB1195). GFAP was detected in rat astrocytes using 10 µg/mL Sheep Anti-Human GFAP Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF2594). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta‑III Tubulin using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for GFAP using the Northern­Lights 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	GFAP in Rat Cortical Stem Cells. GFAP was detected in immersion fixed 7 days differentiated rat cortical stem cells using Sheep Anti-Human GFAP Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF2594) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Human GFAP by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 51 kDa (as indicated) using 0.1 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western
	Detection of Rat GFAP by Simple WesternTM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GFAP at approximately 55 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Rat GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2594) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Glial Fibrillary Acidic Protein
Background	GFAP
background_content	Background: GFAP GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.

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Citations

1. APP upregulation contributes to retinal ganglion cell degeneration via JNK3
   Authors: C Liu, CW Zhang, Y Zhou, WQ Wong, LC Lee, WY Ong, SO Yoon, W Hong, XY Fu, TW Soong, EH Koo, LW Stanton, KL Lim, ZC Xiao, GS Dawe
   Cell Death Differ., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. Time-Dependent, HIV-Tat-Induced Perturbation of Human Neurons In Vitro: Towards a Model for the Molecular Pathology of HIV-Associated Neurocognitive Disorders
   Authors: KT Gurwitz, RJ Burman, BD Murugan, S Garnett, T Ganief, NC Soares, JV Raimondo, JM Blackburn
   Front Mol Neurosci, 2017;10(0):163.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
   BMC Neurosci, 2012;13(0):50.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


4. TNFalpha-induced AMPA-receptor trafficking in CNS neurons; relevance to excitotoxicity?
   Authors: Leonoudakis D, Braithwaite SP, Beattie MS, Beattie EC
   Neuron Glia Biol., 2004;1(3):263-273.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC

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